Material Type  

 학위논문

 

0017161322

Date and Time of Latest Transaction  

20250211151339

ISBN  

9798382835891

DDC  

574

Author  

Eaton, Samuel Anthony.

Title/Author  

Crystallographic and Biochemical Studies of Biosynthetic Enzymes: Using Macromolecular Structure to Understand and Engineer Terpene Biosynthesis.

Publish Info  

[S.l.] : University of Pennsylvania., 2024

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Material Info  

211 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

General Note  

Advisor: Christianson, David W.

학위논문주기  

Thesis (Ph.D.)--University of Pennsylvania, 2024.

Abstracts/Etc  

요약Terpenoid natural products comprise the largest and most structurally diverse class of natural products observed to date. These complex secondary metabolites are synthesized in all domains of life, exhibit potent bioactivities, and are critically important compounds in a number of chemical industries, including the pharmaceutical, energy, and cosmetic industries. Despite the remarkable diversity observed among this class of natural products, all terpenes derive from a mere handful of common primary metabolites, collectively known as isoprenoid diphosphates. Specialized enzymes of secondary metabolism (e.g.; terpenoid cyclases or aromatic prenyltransferases) use these common substrates to generate the diverse hydrocarbon scaffolds that underlie over 102,000 terpenoid natural products. Crystallographic and biochemical studies which illuminate the structure-function relationships of these enzymes help to unravel the complex molecular basis of catalysis. Understanding the structures and chemical mechanisms of biosynthetic enzymes is a crucial step in guiding structure-based engineering approaches to generate new and useful synthetic biology tools. This thesis examines the structural and chemical biology of two microbial terpenoid biosynthetic enzymes, epi-Isozizaene Synthase and Reverse N-Dimethylallyl-L-Tryptophan Synthase 1.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Crystallography

Index Term-Uncontrolled  

Cyclase

Index Term-Uncontrolled  

Enzymology

Index Term-Uncontrolled  

Prenyltransferases

Index Term-Uncontrolled  

Terpenoids

Added Entry-Corporate Name  

University of Pennsylvania Chemistry

Host Item Entry  

Dissertations Abstracts International. 85-12B.

Electronic Location and Access  

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Control Number  

joongbu:658670