자료유형  

 학위논문

Control Number  

0017161461

International Standard Book Number  

9798382843469

Dewey Decimal Classification Number  

581

Main Entry-Personal Name  

Mann, Marina Alexandria.

Publication, Distribution, etc. (Imprint  

[S.l.] : Cornell University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

205 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

General Note  

Includes supplementary digital materials.

General Note  

Advisor: Cilia, Michelle.

Dissertation Note  

Thesis (Ph.D.)--Cornell University, 2024.

Summary, Etc.  

요약Huanglongbing (HLB), also known as citrus greening, poses a significant threat to the citrus industry worldwide, with devastating effects on tree health and fruit production. The disease is primarily spread by the Asian citrus psyllid, Diaphorina citri, which is the vector for the bacterium "Candidatus Liberibacter asiaticus" (CLas), the causal agent of HLB. Long term management of HLB hinges on sustainable control of the vector, reducing transmission of CLas to new, healthy trees. Understanding molecular interactions between D. citri and CLas is crucial for developing effective vector intervention methods. Vector competency, influenced by genetics, environment, and pathogen interactions, determines a vector's ability to transmit a pathogen. In D. citri, vector competency is likely regulated by distinct tissue barriers, which CLas must overcome to be successfully acquired and transmitted. However, understanding the direct barrier-CLas interactions that lead to either successful or failed acquisition and transmission has yet to be determined. In this dissertation, we conducted a comparative transcriptome analysis of healthy and CLas-infected D. citri, focusing on multiple organs including bacteriomes, salivary glands, and heads, in order to track CLas-D. citri interactions throughout the vector body. Using the latest D. citri genome assembly (v3.0), we integrated new transcriptome data with previously published datasets to elucidate the impact of CLas on the transcriptional landscape of D. citri organs involved in circulative, propagative transmission. In order to begin bridging the gap between laboratory-generated transcriptomic results and the reality of wild vector population diversity, we sampled four active citrus groves and assessed hundreds of psyllids for their individual vector competency via population infection rate and CLas titer. Our findings revealed population-level differences in D. citri vector competency, likely influenced by a combination of genetic and environmental factors. From these wild psyllid samples, we aimed to determine if vector competency in D. citri is genetically linked. Previous studies have shown that natural variation in vector capacity is heritable. To assess the genomics of D. citri-CLas interactions, whole genome sequencing of 500 individuals to 7x depth allowed us to associate CLas titer with genomic variability via single nucleotide polymorphisms (SNPs) in a GWAS - one of the first for an agricultural insect vector. We have identified dozens of loci across the genome that make promising targets for interdiction of CLas transmission. The variation in vector competency recorded from both lab and field-sourced samples underscores the importance of assessing multi-trophic pathosystems and disease epidemiology from multiple perspectives. Lessons learned through our vector-pathogen molecular-omics analyses further emphasize this importance. Genome sequencing was foundational for exploring molecular interactions between D. citri and CLas. Further development of omics resources for insect vectors of agriculturally important pathogens is essential.

Subject Added Entry-Topical Term  

Plant pathology.

Subject Added Entry-Topical Term  

Entomology.

Subject Added Entry-Topical Term  

Agriculture.

Subject Added Entry-Topical Term  

Plant sciences.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Asian citrus psyllid

Index Term-Uncontrolled  

GWAS

Index Term-Uncontrolled  

Huanglongbing

Index Term-Uncontrolled  

Psyllids

Index Term-Uncontrolled  

Transcriptomics

Index Term-Uncontrolled  

Vector biology

Added Entry-Corporate Name  

Cornell University Plant Pathology and Plant-Microbe Biology

Host Item Entry  

Dissertations Abstracts International. 85-12B.

Electronic Location and Access  

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Control Number  

joongbu:658289