자료유형  

 학위논문

Control Number  

0017162973

International Standard Book Number  

9798384336495

Dewey Decimal Classification Number  

612

Main Entry-Personal Name  

Vachharajani, Vipul Tushar.

Publication, Distribution, etc. (Imprint  

[S.l.] : Stanford University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

104 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

General Note  

Advisor: Dunn, Alexander.

Dissertation Note  

Thesis (Ph.D.)--Stanford University, 2024.

Summary, Etc.  

요약Cell-cell adhesion and apicobasal polarity are defining characteristics of epithelial tissues that enabled the development of multicellular life. In this thesis, I describe two projects that aim to address questions about each of these fundamental processes.First, I present work using live-cell imaging to study the physical mechanism of lumen formation in Madin-Darby Canine Kidney (MDCK) cell spheroids, a canonical cell-culture model for lumenogenesis. We find that in this system, lumen shape reflects basic geometrical considerations tied to the establishment of apico-basal polarity. A physical model incorporating both cell geometry and intraluminal pressure can account for our observations as well as cases in which pressure plays a dominant role.Next, I present a study characterizing the single-molecule mechanics of an understudied class of cell adhesion molecules. Afadin is an essential actin-binding protein that is associated with both adherens and tight junctions. Using a single-molecule magnetic tweezers assay, we probed the mechanical stability of the bonds between the Afadin PDZ domain and the intracellular domains of two of its binding partners, Nectin-1 and JAM-A. We found that both ligands formed remarkably stable bonds with Afadin-PDZ at forces up to 10 pN. Our data suggest that the relatively-understudied complexes of afadin with JAM-A and Nectin-1 may function in parallel with cadherin-based adhesions to transmit forces across cell-cell junctions.

Subject Added Entry-Topical Term  

Physiology.

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Pathogens.

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Fourier transforms.

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Cell adhesion & migration.

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Disease.

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Permeability.

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Microscopy.

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Extracellular matrix.

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Spheroids.

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Bond strength.

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Morphogenesis.

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Biophysics.

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Cellular biology.

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Developmental biology.

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Mathematics.

Added Entry-Corporate Name  

Stanford University.

Host Item Entry  

Dissertations Abstracts International. 86-03B.

Electronic Location and Access  

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Control Number  

joongbu:657322