자료유형  

 학위논문

Control Number  

0017164608

International Standard Book Number  

9798346874492

Dewey Decimal Classification Number  

540

Main Entry-Personal Name  

Balboa, Samantha J.

Publication, Distribution, etc. (Imprint  

[S.l.] : The University of North Carolina at Chapel Hill., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

125 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-06, Section: B.

General Note  

Advisor: Hicks, Leslie M.;Erie, Dorothy A.

Dissertation Note  

Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2024.

Summary, Etc.  

요약Living organisms respond to stress through a variety of molecular adaptations. These adaptations are critical for survival in hostile environments, including the presence of antibiotics and other stressors. The overall objective of this dissertation is to investigate the mechanisms underlying antimicrobial resistance and stress responses across different biological systems using a combination of proteomics, phenotypic analysis, and metabolomics.The majority of this work focuses on bacterial systems, resistance and -omics technologies to monitor such molecular adaptations to stress. Chapter 2 establishes foundational experimental procedures and analytical strategies for resistance evolution studies and quantitative proteomics. In Chapter 3, the phenotypic consequences of antibiotic resistance were assessed in Escherichia coli, uncovering how resistance reshapes bacterial behavior and phenotype. In Chapter 4, further investigation of proteomic changes in E. coli resistant to the novel AMP CC-AMP1 identified specific proteins and pathways contributing to resistance. In Chapter 5, metabolomic techniques were employed to identify potential biocontrol agents from Pseudomonas protegens to combat Bacterial Panicle Blight in rice, demonstrating the potential of microbial metabolites in agricultural applications. Diverging to a multicellular system, Chapter 6 investigates the role of reversible cysteine oxidation in tardigrade osmobiosis. By labeling free cysteine thiols with a fluorescent probe, separating proteins through two-dimensional electrophoresis, and measuring fluorescence intensity, we observed a decrease in fluorescence signal as tardigrades transitioned into the osmobiotic state, indicating increased cysteine oxidation. Subsequent LC-MS analysis of protein spots with reduced fluorescence provided insights into the adaptive mechanisms that enable extremophiles to survive in hostile environments.Together, these findings provide an improved understanding of the molecular mechanisms that underlie antimicrobial resistance, stress response, and survival, with implications for the development of novel antimicrobial therapies and biocontrol strategies.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Biology.

Subject Added Entry-Topical Term  

Bioinformatics.

Index Term-Uncontrolled  

Antimicrobial resistance

Index Term-Uncontrolled  

Metabolomics

Index Term-Uncontrolled  

Proteomics

Index Term-Uncontrolled  

Stress response

Index Term-Uncontrolled  

Escherichia coli

Added Entry-Corporate Name  

The University of North Carolina at Chapel Hill Chemistry

Host Item Entry  

Dissertations Abstracts International. 86-06B.

Electronic Location and Access  

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Control Number  

joongbu:656675