자료유형  

 학위논문

Control Number  

0017162950

International Standard Book Number  

9798384344797

Dewey Decimal Classification Number  

571.6

Main Entry-Personal Name  

De Los Reyes Labitigan, Ramon Lorenzo.

Publication, Distribution, etc. (Imprint  

[S.l.] : Stanford University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

105 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

General Note  

Includes supplementary digital materials.

General Note  

Advisor: Spudich, James.

Dissertation Note  

Thesis (Ph.D.)--Stanford University, 2024.

Summary, Etc.  

요약The contributions of individual genes to cell-scale morphology and cytoskeletal organization are challenging to define due to the wide intercellular variation of these complex phenotypes. We leveraged the controlled nature of image-based pooled screening to assess the impact of CRISPRi knockdown of 366 genes on cell and nuclear morphology in human U2OS osteosarcoma cells. Screen scale-up was facilitated by a new, efficient barcode readout method that successfully genotyped 85% of cells. Phenotype analysis using a deep learning algorithm, the β-variational autoencoder, produced a feature embedding space distinct from one derived from conventional morphological profiling, but detected similar gene hits while requiring minimal design decisions. We found 45 gene hits and visualized their effect by rationally constrained sampling of cells along the direction of phenotypic shift. By relating these phenotypic shifts to each other, we construct a quantitative and interpretable space of morphological variation in human cells.

Subject Added Entry-Topical Term  

Cells.

Subject Added Entry-Topical Term  

CRISPR.

Subject Added Entry-Topical Term  

Bone cancer.

Subject Added Entry-Topical Term  

Microscopy.

Subject Added Entry-Topical Term  

Medical research.

Subject Added Entry-Topical Term  

Data processing.

Subject Added Entry-Topical Term  

Design.

Subject Added Entry-Topical Term  

Genotype & phenotype.

Subject Added Entry-Topical Term  

Genes.

Subject Added Entry-Topical Term  

Visualization.

Subject Added Entry-Topical Term  

Medical schools.

Subject Added Entry-Topical Term  

Bioengineering.

Subject Added Entry-Topical Term  

Bioinformatics.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Health education.

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Medicine.

Subject Added Entry-Topical Term  

Oncology.

Subject Added Entry-Topical Term  

Education.

Added Entry-Corporate Name  

Stanford University.

Host Item Entry  

Dissertations Abstracts International. 86-03B.

Electronic Location and Access  

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Control Number  

joongbu:655438