자료유형  

 학위논문

Control Number  

0017163970

International Standard Book Number  

9798384081432

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Wong Hickernell, Caitlin Joy.

Publication, Distribution, etc. (Imprint  

[S.l.] : The University of Chicago., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

189 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

General Note  

Advisor: Drummond, David A.

Dissertation Note  

Thesis (Ph.D.)--The University of Chicago, 2024.

Summary, Etc.  

요약When cells experience stress, they undergo a number of dramatic intracellular molecular changes. One of these changes is the formation of biomolecular condensates, or clusters of mRNA and proteins. When these clusters are visible by microscopy, they are called "stress granules." Although these condensates have been observed for many years across many species, many questions remain. What exactly is the composition of a stress granule? What is the mechanism of formation? What, if any, is the function of stress granules? Are they an adaptive response by the cell? The field has not coalesced around answers to these fundamental questions, compelling further study.This dissertation covers a review of stress granules, summarizing previous work and outlining the grand challenges in this area. Additionally, it includes a project on the stress-induced condensation of mRNA. During stress in yeast, we observed global, length-independent condensation of mRNA that seems to be regulated by a block in translation initiation. We also observed that stress-induced transcripts are excluded from condensates, driven by the timing of transcription. Finally, our data suggest the existence of small, submicroscopic condensates that are not visible by standard microscopy. We propose a model where stress-induced mRNA condensation allows the cell to prioritize translation during stress, sequestering away old transcripts so the cell can focus on a stress-specific translational program. Lastly, this work includes a study on whether the stress-induced condensation behavior of poly-A binding protein 1 (Pab1) regulates the translation of stress transcripts during recovery from stress. We did not find data to support this model, leaving Pab1's adaptive condensation behavior as an open question.As a whole, this work comments broadly on stress granules and specifically on how condensation does and does not contribute to the stress response in yeast. It also points toward future challenges and goals, such as a deeper understanding of the mechanism of mRNA condensation and its precise adaptive role during stress.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Biomolecular condensation

Index Term-Uncontrolled  

Heat shock

Index Term-Uncontrolled  

Stress granule

Index Term-Uncontrolled  

Yeast

Added Entry-Corporate Name  

The University of Chicago Biochemistry and Molecular Biophysics

Host Item Entry  

Dissertations Abstracts International. 86-03B.

Electronic Location and Access  

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Control Number  

joongbu:654434