자료유형  

 학위논문

Control Number  

0016932134

International Standard Book Number  

9798379614997

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Lumbroso, Gal.

Publication, Distribution, etc. (Imprint  

[S.l.] : Harvard University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(82 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

General Note  

Advisor: Murray, Andrew W.

Dissertation Note  

Thesis (Ph.D.)--Harvard University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Meiosis is a specialized cell division that includes a round of DNA replication, followed by two rounds of chromosome segregation, producing cells with half the ploidy of the original cell (gametes). The second meiotic division, meiosis II, segregate sister chromatids. The first division, meiosis I, segregates homologous chromosomes (homologs), a process that requires recombination between each homolog pair. Errors in meiosis I chromosome segregation are a frequent source of aneuploidy and reduced gamete viability. In the budding yeast Saccharomyces cerevisiae, diploid cells that commit to meiosis produce four haploid gametes (a tetrad) enveloped in a resilient ascus. We selected for mutations that enable gamete viability in the absence of recombination. Loss-of-function mutations in the B-type cyclin CLB4 arose during that selection. This led us to find that in at least two strains of S. cerevisiae, Clb4 prevents the premature separation of sister chromatids in meiosis I. We show that meiosis in CLB4 deletion mutants from the SK1 and Y55 strain backgrounds often terminates after a single division, in which sister chromatids are segregated instead of homologous chromosomes, demonstrating a novel role of Clb4 in establishing the meiosis I chromosome dynamics and promoting meiotic progression. We reconfirm that in contrast to Y55 and SK1, meiotic progression is largely unperturbed in CLB4 deletion mutants from the W303 strain background, and attempt to explain the genetic basis of this phenotypic difference. 

Subject Added Entry-Topical Term  

Biology.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Cellular biology.

Index Term-Uncontrolled  

Budding yeast

Index Term-Uncontrolled  

CLB4

Index Term-Uncontrolled  

Meiosis

Index Term-Uncontrolled  

Saccharomyces cerevisiae

Index Term-Uncontrolled  

Spo11

Index Term-Uncontrolled  

DNA replication

Added Entry-Corporate Name  

Harvard University Biology Molecular and Cellular

Host Item Entry  

Dissertations Abstracts International. 84-12B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:643918