자료유형  

 학위논문

Control Number  

0016932873

International Standard Book Number  

9798379851811

Dewey Decimal Classification Number  

545

Main Entry-Personal Name  

Pandey, Sangeeta.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2022

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Physical Description  

1 online resource(124 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-01, Section: A.

General Note  

Advisor: Cooks, R. Graham.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Miniature mass spectrometers are in a phase of rapid development due to their potential in offering simple yet powerful solutions for a wide variety of unmet biomedical needs. In particular, the combination of ambient ionization methods with miniature mass spectrometers offers an attractive solution for improving patient outcomes and reducing the healthcare burden on patients as well as clinicians opposed to current methods for disease prognosis and diagnosis.There has been a rapid expansion in the commercial offerings of miniature mass spectrometers from commercial vendors, both large and small, including Purspec, Bayspec, MassTech, Waters, and Advion. Despite the large number of instruments that have been made available and the success of many of these systems with analysis of a broad range of biological matrices, much work remains to perform bioanalysis of complex molecules with concentrations that often lie in the ng/mL-µg/mL range. Miniaturization of mass spectrometers is accompanied by design simplifications in comparison to benchtop instruments, so that sacrifices are often made in terms of performance. The figure of merit that is compromised, of course, depends on the design of the instrument itself. Thus, in addition to a good understanding of the operation of the mass spectrometer, it is crucial that the ionization method for the analyte be chosen judiciously, and that the method is suitably optimized to be able to perform the measurements to obtain high quality data for trace analysis.The custom built Mini-12 miniature MS systems at Purdue University is one such miniature mass spectrometer that can be used for making on-site measurements. In this work, I have described my efforts to perform trace analysis of a range of molecules (tenofovir diphosphate, cabotegravir, rilpivirine, and phosphatidylethanol) relevant to HIV treatment and prevention with the Mini-12 system. Based on the most favorable set of conditions for developing a particular assay, method performance parameters are listed for each of the applications described. In all the above applications, the desired detection limits are met by adopting a broad range of strategies with the focus of keeping the method amenable to use at the point-of-care, i.e., ensuring that sample preparation is completed in 4 minutes. The proof-of-concept results obtained with the custom built Mini-12 mass spectrometer at Purdue University lays the groundwork to (i) encourage the introduction of miniature mass spectrometer-based assays for the molecules listed in a clinical setting and (ii) extend the use of miniature mass spectrometer-based assays for other therapeutic regimens that require longitudinal monitoring.

Subject Added Entry-Topical Term  

Universal Serial Bus.

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Mass spectrometry.

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Software.

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Plasma.

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Human immunodeficiency virus--HIV.

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Arson.

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Power.

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Cellulose.

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Ions.

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Solvents.

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Real time.

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Atmospheric pressure.

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Medical research.

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Drugs.

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Biomarkers.

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Alcohol.

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Vacuum technology.

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Chromatography.

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Blood.

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Metabolites.

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Explosives.

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Interfaces.

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Analytical chemistry.

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Atmospheric sciences.

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Chemistry.

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Criminology.

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Medicine.

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 85-01A.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641920