자료유형  

 학위논문

Control Number  

0016932924

International Standard Book Number  

9798379871963

Dewey Decimal Classification Number  

630

Main Entry-Personal Name  

Hasan, Rifat.

Publication, Distribution, etc. (Imprint  

[S.l.] : North Carolina State University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(223 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-01, Section: B.

General Note  

Advisor: Beck, Ashley;Hall, Steven.

Dissertation Note  

Thesis (Ph.D.)--North Carolina State University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Sustainability in livestock and aquaculture wastewater treatment is essential as these sectors continue to grow and the need for alternative treatments is a pressing concern. This research investigates the potential of the cyanobacterial species S. elongatus UTEX 2973 as an alternative sustainable wastewater treatment to 1) remove inorganic nutrients such as ammonia, nitrate, and phosphate from a combination of swine and fish wastewater while simultaneously accumulating carbohydrate during bioremediation, 2) investigate the phosphate and metal removal by abiotic precipitation and S. elongatus assimilation, and 3) predict growth and nutrient removal using a genome-scale model of S. elongatusUTEX 2973 and compare with experimental findings.The first objective was accomplished by selecting a wastewater mixture from three different mixing ratios (25:75, 50:50, and 75:25) of sturgeon and swine wastewater that supports optimal growth and carbohydrate accumulation in S. elongatus biomass. The combination of 50%-50% sturgeon-swine wastewater was selected for further comparison with BG11 synthetic growth media. A higher growth rate was achieved in synthetic growth media, whereas higher carbohydrate accumulation was observed in mixed wastewater. The nutrient removal efficiencies of S. elongatusin the mixed wastewater were 97.98% for ammonia, 93.39% for nitrate-nitrite, and 67.15% for orthophosphate.Significant phosphate removal by abiotic precipitation was observed due to the high pH (~ 9) of wastewater, which led to the second objective, in which both cyanobacterial and abiotic phosphate removal were studied. It was observed that a total of 96.81% phosphate was removed, of which 80.12% occurred by precipitation and 16.69% by S. elongatusassimilation. Within the first week of the two-week experiment ~ 100% precipitation took place.The final objective was accomplished by performing flux balance analysis (FBA) of a genome-scale model of S. elongatus UTEX 2973 with varying environmental parameters such as carbon dioxide (CO2), ammonia, nitrate, and phosphate to optimize either biomass production or glycogen accumulation (major source of carbohydrates in S. elongatus. No accumulation of glycogen was predicted below CO2 thresholds when examined as a function of inorganic nutrients (ammonia, nitrate, and phosphate), below which no accumulation was predicted. Dynamic flux balance analysis (DFBA) was performed using kinetic parameters for nitrate uptake from literature for another cyanobacterial species, Synechocystis sp. PCC6803. The nutrient uptake rates found experimentally did not fit Michaelis-Menten kinetics due to the complexity of wastewater components. Hence, a regression analysis was performed, and a complete second-order polynomial exhibited a good fit (R2=0.99) with experimentally observed nutrient uptake.The findings of this research showed that S. elongatus could bioremediate mixed wastewater by successfully removing inorganic nutrients and accumulating a significant amount of carbohydrates (70-75% of dry cell mass) during the bioremediation process. This particular species has been minimally investigated before for its ability to transform wastewater nutrients into high-value carbohydrate-enriched biomass. The outcomes of this study can support the development of an S. elongatusbiorefinery approach for bioremediation and manufacturing value-added products.

Subject Added Entry-Topical Term  

Aquaculture.

Subject Added Entry-Topical Term  

Agriculture.

Subject Added Entry-Topical Term  

Nitrates.

Subject Added Entry-Topical Term  

Carbon dioxide.

Subject Added Entry-Topical Term  

Aquatic sciences.

Subject Added Entry-Topical Term  

Biological oceanography.

Added Entry-Corporate Name  

North Carolina State University.

Host Item Entry  

Dissertations Abstracts International. 85-01B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641747