자료유형  

 학위논문

Control Number  

0016932706

International Standard Book Number  

9798379847975

Dewey Decimal Classification Number  

600

Main Entry-Personal Name  

Nkemngong, Carine Ajua.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2021

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2021

Physical Description  

1 online resource(80 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-01, Section: B.

General Note  

Advisor: Oliver, Haley F.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2021.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Pre-wetted disinfectant wipes are increasingly being used to help address the risk of healthcare associated infections (HAIs). However, HAIs are still a major problem in the US with Clostridioides difficile being the most common cause. Dry surface biofilms (DSB) are also major contributors to the HAI problem as they persist on dry environmental surfaces and are harder to kill than wet surface biofilms (WSB) and planktonic bacteria. Limited studies have explored the cross-contamination risk when using disinfectant wipes against C. difficile spores. Even fewer studies have attempted to develop in vitro models for establishing DSB of Staphylococcus aureus and Pseudomonas aeruginosa for disinfectant efficacy testing; as there is currently no official method. The objectives of this work were to (i) determine the cross-contamination risk that prewetted disinfectant towelettes may pose when challenged with C. difficile spores and to; (ii) develop an in vitro model of S. aureus and P. aeruginosaDSB for disinfectant efficacy testing.Methods: Disinfectant wipes were used to wipe a Formica sheet inoculated with C. difficilespores. After the wiping process, log10 CFU on previously uncontaminated pre-determined distances from the inoculation point and on used wipes were determined. In establishing the in vitro DSB model for disinfectant efficacy testing, WSB grown on glass coupons following EPA MLB SOP MB-19 were dehydrated to form DSB and viable bacteria recovered following EPA MLB SOP MB-20.Methods: Disinfectant wipes were used to wipe a Formica sheet inoculated with C. difficile spores. After the wiping process, log10 CFU on previously uncontaminated pre-determined distances from the inoculation point and on used wipes were determined. In establishing the in vitroDSB model for disinfectant efficacy testing, WSB grown on glass coupons following EPA MLB SOP MB-19 were dehydrated to form DSB and viable bacteria recovered following EPA MLB SOP MB-20.Results: Disinfectant wipes transferred C. difficilespores from inoculated surfaces to previously uncontaminated surfaces with hydrogen peroxide (HP) disinfectants being more sporicidal than the quaternary ammonium (QA) disinfectants. A minimum of six mean log10 density of DSB required for disinfectant efficacy against WSB were recovered per coupon through our DSB establishment model. Overall, QA plus alcohol, sodium dichloro-s-triazinetrione and HP products were more bactericidal against DSB than QA products.

Subject Added Entry-Topical Term  

Disinfection & disinfectants.

Subject Added Entry-Topical Term  

Beef.

Subject Added Entry-Topical Term  

Pathogens.

Subject Added Entry-Topical Term  

Ventilators.

Subject Added Entry-Topical Term  

Food contamination & poisoning.

Subject Added Entry-Topical Term  

Salmonella.

Subject Added Entry-Topical Term  

Biofilms.

Subject Added Entry-Topical Term  

Sanitation.

Subject Added Entry-Topical Term  

Mortality.

Subject Added Entry-Topical Term  

Catheters.

Subject Added Entry-Topical Term  

Nosocomial infections.

Subject Added Entry-Topical Term  

Bacteria.

Subject Added Entry-Topical Term  

Staphylococcus infections.

Subject Added Entry-Topical Term  

Hospitals.

Subject Added Entry-Topical Term  

Antimicrobial agents.

Subject Added Entry-Topical Term  

Alcohol.

Subject Added Entry-Topical Term  

Health facilities.

Subject Added Entry-Topical Term  

Illnesses.

Subject Added Entry-Topical Term  

Yeast.

Subject Added Entry-Topical Term  

Microorganisms.

Subject Added Entry-Topical Term  

Dairy products.

Subject Added Entry-Topical Term  

Hydrogen peroxide.

Subject Added Entry-Topical Term  

Food science.

Subject Added Entry-Topical Term  

Medicine.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Pathology.

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 85-01B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641529