자료유형  

 학위논문

Control Number  

0016933762

International Standard Book Number  

9798380262132

Dewey Decimal Classification Number  

616

Main Entry-Personal Name  

Fowler, Erin.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of Pittsburgh., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(34 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

General Note  

Advisor: Mailliard, Robbie;Mattila, Joshua;Doi, Yohei.

Dissertation Note  

Thesis (M.Sc.)--University of Pittsburgh, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Klebsiella pneumoniae is one of the most common causes of hospital-acquired infections, and is becoming increasingly resistant to various agents including colistin, a last resort antibiotic. As there are limited treatment options for these types of infections, bacteriophages active against them are drawing attention as a potential treatment option. To identify such bacteriophages, environmental water samples were screened against five different colistin-resistant clinical strains. As a result, two individual bacteriophages, MC8 and MC9, with activity against a colistin-resistant Klebsiella pneumoniae strain were isolated. Both bacteriophages were exclusively active againstK. pneumoniae Sequence Type (ST) 258 clade I strains, except for MC9, which was also active against an additional K. pneumoniae ST433 strain. Sequence analysis of the two bacteriophages showed identical sequences except for a 52 base pair nucleotide deletion in the tail spike gene in MC9. Generation of phage-resistant mutants to each bacteriophage was performed to identify their targets. Upon analysis of the phage-resistant mutant sequences, an insertion sequence was found in the wcaJgene encoding undecaprenyl-phosphate glucose-1-phosphage transferase. This gene helps catalyze the synthesis of colanic acid, a polysaccharide in the extracellular membrane, and a known attachment point used for phage entry.

Subject Added Entry-Topical Term  

Genetic testing.

Subject Added Entry-Topical Term  

Infections.

Subject Added Entry-Topical Term  

Infectious diseases.

Subject Added Entry-Topical Term  

Pathogens.

Subject Added Entry-Topical Term  

Antibiotics.

Subject Added Entry-Topical Term  

Mutation.

Subject Added Entry-Topical Term  

Bacterial infections.

Subject Added Entry-Topical Term  

Bacteria.

Subject Added Entry-Topical Term  

Lawns.

Subject Added Entry-Topical Term  

Fatty acids.

Subject Added Entry-Topical Term  

Glucose.

Subject Added Entry-Topical Term  

Public health.

Subject Added Entry-Topical Term  

Genomes.

Subject Added Entry-Topical Term  

Biology.

Subject Added Entry-Topical Term  

Protozoa.

Subject Added Entry-Topical Term  

Gram-negative bacteria.

Subject Added Entry-Topical Term  

Research & development--R&D.

Subject Added Entry-Topical Term  

Enzymes.

Subject Added Entry-Topical Term  

Drug resistance.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Pathology.

Subject Added Entry-Topical Term  

Pharmaceutical sciences.

Subject Added Entry-Topical Term  

Pharmacology.

Added Entry-Corporate Name  

University of Pittsburgh.

Host Item Entry  

Dissertations Abstracts International. 85-03B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641456