자료유형  

 학위논문

Control Number  

0016933807

International Standard Book Number  

9798380264631

Dewey Decimal Classification Number  

616

Main Entry-Personal Name  

Jones, Chey Marcel.

Publication, Distribution, etc. (Imprint  

[S.l.] : Stanford University., 2022

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Physical Description  

1 online resource(266 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

General Note  

Advisor: Boxer, Steven G.;Markland, Thomas E.;Martinez, Todd.

Dissertation Note  

Thesis (Ph.D.)--Stanford University, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약The green fluorescent protein (GFP) has revolutionized the fields of chemistry and biology. Due to their ability to highlight protein dynamics in living organisms, GFP and GFP-like proteins have emerged as ubiquitous tools in biological imaging. The versatility of GFP has inspired a rainbow-like palette of fluorescent reporters; however, the creation of novel GFP variants is often imprecise and inefficient. Using molecular dynamics simulations, I unravel the intricate steric and electronic factors responsible for the performance of these fluorescent proteins. Across many systems (i.e., gas phase, water, and GFP/Dronpa2 scaffolds), I study the photodynamics of the GFP chromophore. Changes to the chromophore's intrinsic properties (e.g.fluorescence propensity, photoisomerization quantum yield, etc.) are monitored as the molecule occupies various condensed phase systems. Applying this knowledge, I have developed a computational workflow that can (1) generate structures for tens of thousands of GFP mutants and (2) characterize these mutants using steric and electronic features. This structural data is coupled with machine learning models to identify important interactions within the system and inspire the creation of novel GFP reporters.

Subject Added Entry-Topical Term  

Cancer.

Subject Added Entry-Topical Term  

Software.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Mutation.

Subject Added Entry-Topical Term  

Microscopy.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Amino acids.

Subject Added Entry-Topical Term  

E coli.

Subject Added Entry-Topical Term  

Energy.

Subject Added Entry-Topical Term  

Mutagenesis.

Subject Added Entry-Topical Term  

Genetics.

Added Entry-Corporate Name  

Stanford University.

Host Item Entry  

Dissertations Abstracts International. 85-03B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641311