자료유형  

 학위논문

Control Number  

0016931778

International Standard Book Number  

9798379720520

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Romer, Brianna Lynne.

Publication, Distribution, etc. (Imprint  

[S.l.] : Princeton University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(110 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

General Note  

Advisor: Petry, Sabine.

Dissertation Note  

Thesis (Ph.D.)--Princeton University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Microtubules (MTs) are dynamic cylindrical polymers made of α/β-tubulin dimers that function in many diverse and essential cellular processes such as cell division, intracellular transport, and organelle positioning. Because of their importance, it is imperative that microtubules are nucleated at the correct time and place. A protein known to be essential for MT nucleation is γ-tubulin, which together with other proteins in the cell, forms a ring-like template called the γ-tubulin ring complex (γ-TuRC). Despite its importance, little is known about how the γ-TuRC nucleates MTs. For many years, it has been proposed that the γ-TuRC undergoes a large structural change upon activation, though this has not been observed directly. In this dissertation, I present two complementary structures of the γ-TuRC. The first structure is a cryo-electron microscopy (cryo-EM) structure of the γ-TuRC with an activating protein domain, which shows dynamic movement within the complex. While theorized for many years, I present the first example of structural changes that occur to change the diameter of the γ-TuRC. The second structure is the first cryo-electron tomography (cryo-ET) sub-tomogram average of the γ-TuRC in a post-nucleation state. This reconstruction of the γ-TuRC bound to a MT provides insight into the position of the γ-TuRC in relation to a newly formed MT. Together, my work helps to further our understanding of how the universal MT nucleator, the γ-TuRC, functions to regulate the production of MTs.

Subject Added Entry-Topical Term  

Molecular biology.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Cellular biology.

Index Term-Uncontrolled  

Cryo-electron microscopy

Index Term-Uncontrolled  

Cryo-electron tomography

Index Term-Uncontrolled  

Gamma-tubulin ring complex

Index Term-Uncontrolled  

Microtubule

Index Term-Uncontrolled  

Cylindrical polymers

Added Entry-Corporate Name  

Princeton University Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 84-12B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:639486