Material Type  

 학위논문

 

0017160617

Date and Time of Latest Transaction  

20250211151049

ISBN  

9798382840130

DDC  

620.8

Author  

Mora-Antoinette, Macy.

Title/Author  

The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation.

Publish Info  

[S.l.] : Cornell University., 2024

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Material Info  

142 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

General Note  

Advisor: Lewis, Karl.

학위논문주기  

Thesis (Ph.D.)--Cornell University, 2024.

Abstracts/Etc  

요약Bone's ability to adapt to mechanical loads is attributed to the osteocyte. Osteocytes reside embedded in the calcified bone matrix, where they function as the resident tissue mechanotransducer. Calcium (Ca2+) signaling is a key second messenger in osteocyte mechanotransduction, however the details regarding Ca2+ signaling regulation in osteocytes in vivo are not well stratified. The nervous system is known to regulate bone. Osteocytes express components for acetylcholine (ACh) receptors that are known for rapid firing at the neuromuscular junction. Although ACh is known to impact bone mass and fracture risk, the details regarding ACh signaling in bone mechanotransduction remain largely unexplored.This talk identifies osteocytes as a functional target for cholinergic signaling with impacts on bone mechanoadaptation and Ca2+ signaling. We generated osteocyte-targeted conditional knockout mice to remove key components used by ACh receptors. We assessed tissue material make-up, strength, and formations rates to anabolic loading. We also used intravital imaging via two-photon fluorescent microscopy to visualize the Ca2+ dynamics in osteocytes during active mechanical loading of the bone in living mice. Finally, we explore the ability of light-sheet fluorescent microscopy to create high resolution 3D reconstructions of nerve bodies in whole-mount and intact bone specimens. Our results establish a new signaling axis between the brain and bone via the osteocyte with far reaching implications in bone biology which may be vital to treat bone diseases.

Subject Added Entry-Topical Term  

Biomechanics.

Subject Added Entry-Topical Term  

Physiology.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Neurosciences.

Index Term-Uncontrolled  

Bone

Index Term-Uncontrolled  

Calcium signaling

Index Term-Uncontrolled  

Imaging

Index Term-Uncontrolled  

Mechanoadaptation

Index Term-Uncontrolled  

Nicotinic acetylcholine receptors

Index Term-Uncontrolled  

Osteocytes

Added Entry-Corporate Name  

Cornell University Mechanical Engineering

Host Item Entry  

Dissertations Abstracts International. 85-12B.

Electronic Location and Access  

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Control Number  

joongbu:658586