자료유형  

 학위논문

Control Number  

0017160101

International Standard Book Number  

9798384446675

Dewey Decimal Classification Number  

615.9

Main Entry-Personal Name  

Lu, Sixin Samantha.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, Berkeley., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

65 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

General Note  

Advisor: Rappaport, Stephen M.

Dissertation Note  

Thesis (Ph.D.)--University of California, Berkeley, 2024.

Summary, Etc.  

요약Ambient air pollution and household air pollution are among the leading causes of mortality, responsible for 6 million premature deaths per year globally. The combustion of fossil fuels, including diesel and coal, constitutes a major source of air pollution. Polycyclic aromatic hydrocarbons (PAHs) are a group of compounds released during incomplete combustion of organic material which has been classified as carcinogenic to humans. Inhalational exposure to combustion emissions can be quantitated either externally such as by determining personal airborne PAH levels, or internally by determining the levels of a biomarker in the exposed population. Given the ease of sample collection, identifying suitable urine and blood biomarkers that accurately reflect exposure to air pollution is of significant interest. This dissertation examines the relations of the levels of biomarkers in urine and plasma with exposure determinants and with personal airborne measurements in humans exposed to emissions from diesel and solid fuel combustion. First, urinary PAH levels were measured using headspace solid-phase microextraction and gas chromatography-mass spectrometry in 28 nonsmokers in two studies before, immediately after and the day after short-term, controlled exposures to diesel exhaust (DE). Likelihood-ratio tests on linear mixed-effects models indicated that, after adjusting for other covariates, DE exposure did not significantly alter urinary PAH levels. Second, urinary PAH levels were reported in 163 Chinese female nonsmokers from Xuanwei and Fuyuan counties, where the highest lung cancer rates in the world are observed due to household air pollution from combustion of smoky coal. Mixed-effects models revealed that personal airborne PAH levels (over a wide range of exposure up to occupational levels experienced by coke oven workers), age and exposure characteristics such as fuel and stove types were significant predictors of urinary PAH levels. Third, using an untargeted nanoflow liquid chromatography and high-resolution mass spectrometry method, 50 adducts were detected at the nucleophilic Cys34 locus of human serum albumin (HSA) in 29 of the aforementioned Xuanwei and Fuyuan women and 10 local controls. Upon adduct annotation and quantitation, Wilcoxon rank sum tests showed that levels of S-glutathione (S-GSH) and S-γ-glutamylcysteine (S-γ-GluCys), adducts related to glutathione, an important antioxidant, were lower in exposed subjects compared to those in controls. After adjustment for age and personal measurements of airborne benzo(a)pyrene (a representative carcinogenic PAH) using multivariate regressions, the largest reductions in levels of S-GSH and S-γ-GluCys relative to controls were observed for smoky coal users, compared to users of smokeless coal and wood. These studies collectively demonstrate that urinary unmetabolized PAHs may be suitable biomarkers of wide-ranging exposures to fuel combustion emissions and that Cys34 adductomics is a promising tool for discovering biomarkers of longer-term exposures, owing to the one-month residence time of HSA.

Subject Added Entry-Topical Term  

Toxicology.

Subject Added Entry-Topical Term  

Environmental health.

Subject Added Entry-Topical Term  

Public health.

Index Term-Uncontrolled  

Biomarkers

Index Term-Uncontrolled  

Diesel exhaust

Index Term-Uncontrolled  

Air pollution

Index Term-Uncontrolled  

Human serum albumin

Index Term-Uncontrolled  

Polycyclic aromatic hydrocarbons

Index Term-Uncontrolled  

Smoky coal

Index Term-Uncontrolled  

Urine

Added Entry-Corporate Name  

University of California, Berkeley Molecular Toxicology

Host Item Entry  

Dissertations Abstracts International. 86-03B.

Electronic Location and Access  

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Control Number  

joongbu:658124