자료유형  

 학위논문

Control Number  

0017162250

International Standard Book Number  

9798383225530

Dewey Decimal Classification Number  

576

Main Entry-Personal Name  

Kargol, Abigail.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of Washington., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

147 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-01, Section: B.

General Note  

Advisor: Gough, Heidi.

Dissertation Note  

Thesis (Ph.D.)--University of Washington, 2024.

Summary, Etc.  

요약As demand for clean water increases, it is important to employ strategies to preserve both water quality and quantity. One strategy is the use of treated wastewater for irrigation, which can also serve as a wastewater treatment mechanism. Poplar trees and associated soil microorganisms remove pollutants from the irrigation water and the nutrient inputs increase crop growth. While treatment performance in wastewater infiltration systems has been studied, the contribution of the soil microbial community is not well-understood. We aimed to study nitrogen removal performance and biomass production in a poplar-planted wastewater infiltration system and assess impacts on microbial community structure and function. Reactors, along with bare-soil controls, were irrigated with synthetic wastewater effluent with increasing nitrogen concentrations for 18 months. Experiments were conducted each season, and soil samples were collected after each experiment. Soil DNA extracts were sequenced with 16S third-generation nanopore sequencing to examine community composition and droplet digital PCR was used to quantify key nitrogen cycling genes. Across all seasons, significant nitrate and total nitrogen removal was observed in planted reactors, while performance of unplanted reactors decreased in the winter months and under high nitrogen loads, highlighting the importance of poplars in taking up nitrogen and providing organic carbon. Microbial community diversity was not impacted by exposure to low-strength wastewater, but statistical visualization suggested differences in key microbial groups between reactors with and without trees. Nitrogen cycling in planted and unplanted soils was altered by wastewater. Nitrification was impacted at the gene level, with wastewater irrigated reactors having higher abundance of amoA, while denitrification was impacted at the level of activity, with increased denitrification potential in soils treated with higher nitrogen and organic matter loads. Compared to control reactors receiving tap water, poplars irrigated with wastewater produced 3 times more aboveground biomass, and an economic evaluation showed that establishing tertiary treatment wastewater infiltration systems for bioenergy production and water recovery (WISER) could produce substantial profits for wastewater treatment facilities via the sale of recovered water. These results supported the potential for year-round operation of infiltration systems for treatment and biomass production and highlighted the importance of vegetation for optimal treatment performance. Impacts to the soil community were minimal and served to enhance treatment without impacting overall community structure and function. This work will help inform the design and operation of WISER, providing opportunities for inexpensive tertiary treatment and the expansion of the bioeconomy.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Nanoscience.

Subject Added Entry-Topical Term  

Nutrition.

Index Term-Uncontrolled  

Hybrid poplar

Index Term-Uncontrolled  

Nanopore sequencing

Index Term-Uncontrolled  

Nutrient recovery

Index Term-Uncontrolled  

Wastewater

Index Term-Uncontrolled  

Nitrification

Added Entry-Corporate Name  

University of Washington Environmental and Forest Science

Host Item Entry  

Dissertations Abstracts International. 86-01B.

Electronic Location and Access  

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Control Number  

joongbu:657907