자료유형  

 학위논문

Control Number  

0017162873

International Standard Book Number  

9798382741178

Dewey Decimal Classification Number  

574.191

Main Entry-Personal Name  

Wang, Shiyuan.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of Michigan., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

125 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

General Note  

Advisor: Yang, Qiong.

Dissertation Note  

Thesis (Ph.D.)--University of Michigan, 2024.

Summary, Etc.  

요약This dissertation aims to investigate the properties of the mitotic oscillator and its complex regulatory mechanism. Responsible for the progression of the cell cycle through its various stages, the mitotic oscillator is highly conserved in organisms and is essential for life, however the design principles of the biochemical network behind the oscillator and the significance of its topology are not fully understood. Falling into the "activator-inhibitor" category, the mitotic circuit has a negative-plus-positive feedback structure. To untangle the complex interactions of this circuit, in this work I present a novel droplet-based platform combined with microfluidic techniques where Xenopus egg extracts were encapsulated in water-in-oil microemulsions. Our system allows for the fine tuning of all kinds of variables including droplet size and extract content and can reliably reconstitute the cell cycle dynamics. Primary tests using this system indicate that the mitotic oscillators are to a certain extent robust to perturbations while tunable in bi-stability, speed, and accuracy. Experimentations on partial inhibition of the network revealed shortcomings of current cell cycle models, which cannot explain the oscillator's multimodal behavior and thus suggests other essential regulatory pathways. Taking advantage of the setup. I also investigated the oscillator behavior at different energy levels and discovered a non-monotonic response in oscillator speed and accuracy, which points to interesting mechanisms designed to sense and respond to a cell's energy budget.

Subject Added Entry-Topical Term  

Biophysics.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Physical chemistry.

Index Term-Uncontrolled  

Biological oscillators

Index Term-Uncontrolled  

Microfluidics

Index Term-Uncontrolled  

Tunability

Index Term-Uncontrolled  

Mitotic oscillator

Added Entry-Corporate Name  

University of Michigan Biophysics

Host Item Entry  

Dissertations Abstracts International. 85-12B.

Electronic Location and Access  

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Control Number  

joongbu:657761