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Innovations for Improved Chemical Imaging and Optical Manipulation in Biological Systems.
Innovations for Improved Chemical Imaging and Optical Manipulation in Biological Systems.
상세정보
- 자료유형
- 학위논문
- Control Number
- 0017161526
- International Standard Book Number
- 9798342104531
- Dewey Decimal Classification Number
- 535
- Main Entry-Personal Name
- Clark, Matthew Graham.
- Publication, Distribution, etc. (Imprint
- [S.l.] : Purdue University., 2024
- Publication, Distribution, etc. (Imprint
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- Physical Description
- 127 p.
- General Note
- Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
- General Note
- Advisor: Zhang, Chi.
- Dissertation Note
- Thesis (Ph.D.)--Purdue University, 2024.
- Summary, Etc.
- 요약Chemical imaging is a ubiquitous approach to mapping out the complex and dynamic biochemical landscapes of living systems. Here, chemically-specific optical microscopy allows for reaction specific monitoring at sub-cellular levels of resolution. However, the ability to monitor chemical reactions only gives the analyst a passive view of cellular activities. To better understand these processes, it is important to develop new methods for controlling chemical reactions. Compared to conventional methods, optical methods for chemical manipulation provide unprecedented spatial and temporal control and can be integrated into the same imaging platform. In this work, we demonstrate the development of a novel integrated imaging and opto-control platform for highly chemically specific imaging and for spatial and temporally specific laser manipulation.The design of the imaging platform uses nonlinear optical techniques such as coherent Raman scattering and multiphoton excitation fluorescence for maximizing chemical selection criteria for potential reaction monitoring. The key issue with the integration of these techniques into a single platform is the excitation scheme, typically picosecond lasers are used for Raman and femtosecond for fluorescence. To combat this, we developed a pulse-picking device that can tune the peak power of the excitation lasers while maintaining low average powers on sample. This minimizes phototoxicity and generates higher nonlinear optical signals. This device allows for a seamless integration of imaging techniques and allows for faster dwell times to be used during laser scanning.We further develop the imaging platform by implementing optical control lasers into the microscopy setup. Using the signals generated during laser scanning, we compare the voltage readout from the detector and compare it with an existing threshold we set in a lab-designed comparator circuit. If the voltage set is greater than the threshold, we use a voltage output from the circuit to turn the control lasers on .This allows us to create a feedback loop that makes realtime decision on when and where to direct the laser. We demonstrate further capability of decision making through bandpass selection and digital logic functions which can be designed at the user's choice. This approach gives a generic approach to chemical monitoring and optical control at time and length scales appropriate for making meaningful biochemical measurements in dynamic systems.
- Subject Added Entry-Topical Term
- Spectrum analysis.
- Subject Added Entry-Topical Term
- Lasers.
- Subject Added Entry-Topical Term
- Power.
- Subject Added Entry-Topical Term
- Chemical reactions.
- Subject Added Entry-Topical Term
- Microscopy.
- Subject Added Entry-Topical Term
- Labeling.
- Subject Added Entry-Topical Term
- Polymethyl methacrylate.
- Subject Added Entry-Topical Term
- Design.
- Subject Added Entry-Topical Term
- Energy.
- Subject Added Entry-Topical Term
- Lipids.
- Subject Added Entry-Topical Term
- Transistors.
- Subject Added Entry-Topical Term
- Optics.
- Subject Added Entry-Topical Term
- Atoms & subatomic particles.
- Subject Added Entry-Topical Term
- Vibration.
- Subject Added Entry-Topical Term
- Analytical chemistry.
- Subject Added Entry-Topical Term
- Atomic physics.
- Subject Added Entry-Topical Term
- Polymer chemistry.
- Added Entry-Corporate Name
- Purdue University.
- Host Item Entry
- Dissertations Abstracts International. 86-04B.
- Electronic Location and Access
- 로그인을 한후 보실 수 있는 자료입니다.
- Control Number
- joongbu:657706
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798342104531
■035 ▼a(MiAaPQ)AAI31285281
■035 ▼a(MiAaPQ)25395811
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a535
■1001 ▼aClark, Matthew Graham.
■24510▼aInnovations for Improved Chemical Imaging and Optical Manipulation in Biological Systems.
■260 ▼a[S.l.]▼bPurdue University. ▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a127 p.
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-04, Section: B.
■500 ▼aAdvisor: Zhang, Chi.
■5021 ▼aThesis (Ph.D.)--Purdue University, 2024.
■520 ▼aChemical imaging is a ubiquitous approach to mapping out the complex and dynamic biochemical landscapes of living systems. Here, chemically-specific optical microscopy allows for reaction specific monitoring at sub-cellular levels of resolution. However, the ability to monitor chemical reactions only gives the analyst a passive view of cellular activities. To better understand these processes, it is important to develop new methods for controlling chemical reactions. Compared to conventional methods, optical methods for chemical manipulation provide unprecedented spatial and temporal control and can be integrated into the same imaging platform. In this work, we demonstrate the development of a novel integrated imaging and opto-control platform for highly chemically specific imaging and for spatial and temporally specific laser manipulation.The design of the imaging platform uses nonlinear optical techniques such as coherent Raman scattering and multiphoton excitation fluorescence for maximizing chemical selection criteria for potential reaction monitoring. The key issue with the integration of these techniques into a single platform is the excitation scheme, typically picosecond lasers are used for Raman and femtosecond for fluorescence. To combat this, we developed a pulse-picking device that can tune the peak power of the excitation lasers while maintaining low average powers on sample. This minimizes phototoxicity and generates higher nonlinear optical signals. This device allows for a seamless integration of imaging techniques and allows for faster dwell times to be used during laser scanning.We further develop the imaging platform by implementing optical control lasers into the microscopy setup. Using the signals generated during laser scanning, we compare the voltage readout from the detector and compare it with an existing threshold we set in a lab-designed comparator circuit. If the voltage set is greater than the threshold, we use a voltage output from the circuit to turn the control lasers on .This allows us to create a feedback loop that makes realtime decision on when and where to direct the laser. We demonstrate further capability of decision making through bandpass selection and digital logic functions which can be designed at the user's choice. This approach gives a generic approach to chemical monitoring and optical control at time and length scales appropriate for making meaningful biochemical measurements in dynamic systems.
■590 ▼aSchool code: 0183.
■650 4▼aSpectrum analysis.
■650 4▼aLasers.
■650 4▼aPower.
■650 4▼aChemical reactions.
■650 4▼aMicroscopy.
■650 4▼aLabeling.
■650 4▼aPolymethyl methacrylate.
■650 4▼aDesign.
■650 4▼aEnergy.
■650 4▼aLipids.
■650 4▼aTransistors.
■650 4▼aOptics.
■650 4▼aAtoms & subatomic particles.
■650 4▼aVibration.
■650 4▼aAnalytical chemistry.
■650 4▼aAtomic physics.
■650 4▼aPolymer chemistry.
■690 ▼a0791
■690 ▼a0389
■690 ▼a0752
■690 ▼a0486
■690 ▼a0748
■690 ▼a0495
■71020▼aPurdue University.
■7730 ▼tDissertations Abstracts International▼g86-04B.
■790 ▼a0183
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161526▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
