자료유형  

 학위논문

Control Number  

0017161526

International Standard Book Number  

9798342104531

Dewey Decimal Classification Number  

535

Main Entry-Personal Name  

Clark, Matthew Graham.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

127 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-04, Section: B.

General Note  

Advisor: Zhang, Chi.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2024.

Summary, Etc.  

요약Chemical imaging is a ubiquitous approach to mapping out the complex and dynamic biochemical landscapes of living systems. Here, chemically-specific optical microscopy allows for reaction specific monitoring at sub-cellular levels of resolution. However, the ability to monitor chemical reactions only gives the analyst a passive view of cellular activities. To better understand these processes, it is important to develop new methods for controlling chemical reactions. Compared to conventional methods, optical methods for chemical manipulation provide unprecedented spatial and temporal control and can be integrated into the same imaging platform. In this work, we demonstrate the development of a novel integrated imaging and opto-control platform for highly chemically specific imaging and for spatial and temporally specific laser manipulation.The design of the imaging platform uses nonlinear optical techniques such as coherent Raman scattering and multiphoton excitation fluorescence for maximizing chemical selection criteria for potential reaction monitoring. The key issue with the integration of these techniques into a single platform is the excitation scheme, typically picosecond lasers are used for Raman and femtosecond for fluorescence. To combat this, we developed a pulse-picking device that can tune the peak power of the excitation lasers while maintaining low average powers on sample. This minimizes phototoxicity and generates higher nonlinear optical signals. This device allows for a seamless integration of imaging techniques and allows for faster dwell times to be used during laser scanning.We further develop the imaging platform by implementing optical control lasers into the microscopy setup. Using the signals generated during laser scanning, we compare the voltage readout from the detector and compare it with an existing threshold we set in a lab-designed comparator circuit. If the voltage set is greater than the threshold, we use a voltage output from the circuit to turn the control lasers on .This allows us to create a feedback loop that makes realtime decision on when and where to direct the laser. We demonstrate further capability of decision making through bandpass selection and digital logic functions which can be designed at the user's choice. This approach gives a generic approach to chemical monitoring and optical control at time and length scales appropriate for making meaningful biochemical measurements in dynamic systems.

Subject Added Entry-Topical Term  

Spectrum analysis.

Subject Added Entry-Topical Term  

Lasers.

Subject Added Entry-Topical Term  

Power.

Subject Added Entry-Topical Term  

Chemical reactions.

Subject Added Entry-Topical Term  

Microscopy.

Subject Added Entry-Topical Term  

Labeling.

Subject Added Entry-Topical Term  

Polymethyl methacrylate.

Subject Added Entry-Topical Term  

Design.

Subject Added Entry-Topical Term  

Energy.

Subject Added Entry-Topical Term  

Lipids.

Subject Added Entry-Topical Term  

Transistors.

Subject Added Entry-Topical Term  

Optics.

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Atoms & subatomic particles.

Subject Added Entry-Topical Term  

Vibration.

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Analytical chemistry.

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Atomic physics.

Subject Added Entry-Topical Term  

Polymer chemistry.

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 86-04B.

Electronic Location and Access  

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Control Number  

joongbu:657706