자료유형  

 학위논문

Control Number  

0017164295

International Standard Book Number  

9798342143080

Dewey Decimal Classification Number  

664.12

Main Entry-Personal Name  

Nsiah, Sarah Twumwah.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

160 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-04, Section: B.

General Note  

Advisor: McLuckey, Scott A.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2024.

Summary, Etc.  

요약Different ion type formation has been explored using ion-ion chemistry in the gas-phase. With the many advantages of gas-phase reactions, tandem mass spectrometry and the flexibility in selecting reagents and analytes in modified instruments for ion-ion reactions, the possibility of exploring reactions that most likely result in the formation of desirable product(s) and fragments seem endless.In this thesis, the formation of desirable ion types for lipid structural analysis and chemical reactions is discussed.Chapter 1 focuses on some of the gas phase techniques and methodologies used in generating the data presented in this thesis - introduction to mass spectrometry, electrospray ionization, tandem mass spectrometry, ion-ion reactions and lipid classes. Chapters 2 to 4 focuses on the use of charge inversion for structural elucidation of lipids. In the absence of a chemical derivatization fatty acid (FA) anions do not yield all of the structural information that may be of interest under commonly used collision-induced dissociation (CID) conditions. Derivatization in the solution phase presents downsides that can be overcome in the gas phase. Therefore, gas-phase ion/ion chemistry to charge invert complex lipids and fatty acid anions to an ion-type that is structurally informative when conventional CID is employed is desirable. The various chapters in this dissertation cover topics focused on making desirable ions. Most of the focus is on lipid structural characterization, motivated by the potential use of metal ligand complexes for significant improvement in the overall efficiency of generating structural information and for differentiating isomers. A hybrid triple quadrupole/linear ion trap tandem mass spectrometer and a TripleTOF instrument that has been modified to enable the execution of ion/ion reaction experiments was used for the experiments.Chapter 2 covers the use of 4,4',4"-tri-tert-butyl-2,2':6',2"-terpyridine (ttb-Terpy) for 19 improved efficiency in FA characterization. Chapter 3 focuses on differentiating isomers of complex phosphatidylglycerol (PG) and bis(monoacylglycero)phosphate (BMP) lipids using gas phase charge inversion reactions.Chapter 4 provides information on the use of 4'-nitro-2,2':6',2"- terpyridine (NO2-Terpy) ligand for improved efficiency in FA characterization. In chapter 5, the focus is drawn away from lipid structural characterization to the use of ion-ion chemistry for chemical reactions in a molecular container. It focuses on chemical reactions entirely in the gas phase using molecular containersfor bond formations between ions of the same polarity. As proofof-concept, PAMAM 3.5G dendrimers are used as molecular containers for bond formation between sugars. Covalent bond formation is also shown using peptides.

Subject Added Entry-Topical Term  

Sucrose.

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Mass spectrometry.

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Spleen.

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Glycerol.

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Hydrocarbons.

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Electrons.

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Reagents.

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Electric fields.

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Chemical reactions.

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Solvents.

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Fatty acids.

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Glucose.

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Molecular weight.

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Scientific imaging.

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Chemical bonds.

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Chromatography.

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Lipids.

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Analytical chemistry.

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Atomic physics.

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Electromagnetics.

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 86-04B.

Electronic Location and Access  

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Control Number  

joongbu:657662