자료유형  

 학위논문

Control Number  

0017163707

International Standard Book Number  

9798342113441

Dewey Decimal Classification Number  

600

Main Entry-Personal Name  

Benjamin, Kaisha Nekesa.

Publication, Distribution, etc. (Imprint  

[S.l.] : Stanford University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

162 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-04, Section: B.

General Note  

Advisor: Endy, Drew.

Dissertation Note  

Thesis (Ph.D.)--Stanford University, 2024.

Summary, Etc.  

요약Diabetes is a chronic endocrine disease characterized by insulin deficiency and resulting elevated blood glucose levels. Diabetics depend on exogenous injections of insulin to manage blood glucose levels and survive longer term. Poorly controlled diabetes can result in heart disease, neuropathy, and kidney failure. As a potential alternative, I worked towards the development of a commensal skin microbe, Staphylococcus epidermidis, engineered to sense and respond to elevated blood glucose levels. The ultimate goal is to implement within S. epidermidis a transcription-based biosensor responsive to elevated blood sugar levels that results in well-regulated and rapid production of a single-chain insulin analog. Here, I quantitatively estimated whether a skin microbe could provide enough insulin to treat Type I or Type II diabetes. I then developed a transcriptomics pipeline for characterizing organism-wide gene expression changes in response to medically relevant glucose concentrations, to facilitate the identification of glucose-inducible S. epidermidis switches. I also quantitatively explored the genome-wide transcription response of S. epidermidis to heat shock, both as a reference case for glucose response and its own merits. Finally, I explored whether conjugation could be used to reliably transfer DNA into S. epidermidis.

Subject Added Entry-Topical Term  

Infectious diseases.

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Physiology.

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Hair.

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Pathogens.

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Diabetes.

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Bacteria.

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Skin diseases.

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Chemistry.

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Metabolism.

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Teachers.

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Homeostasis.

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Synthetic biology.

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Bioengineering.

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Genetic engineering.

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Clinical trials.

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Glucose.

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Viruses.

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Chemical bonds.

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Phenylketonuria.

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Acne.

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Pathogenesis.

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Metabolites.

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Biology.

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Dermatology.

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Genetics.

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Medicine.

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Pathology.

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Pharmaceutical sciences.

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Public health.

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Virology.

Added Entry-Corporate Name  

Stanford University.

Host Item Entry  

Dissertations Abstracts International. 86-04B.

Electronic Location and Access  

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Control Number  

joongbu:657574