자료유형  

 학위논문

Control Number  

0017165162

International Standard Book Number  

9798346830252

Dewey Decimal Classification Number  

541.3

Main Entry-Personal Name  

Ma, Hui.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

176 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-06, Section: B.

General Note  

Advisor: Linnes, Jacqueline C.;Kinzer-Ursem, Tamara L.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2024.

Summary, Etc.  

요약Microfluidic techniques have been widely adopted in biomedical research due to the precise control of fluids, small volume requirement, low cost and etc, and have boosted the development of biomolecular interaction analysis, point-of-care diagnostics, and biosensors.Protein-protein interaction plays a key role in biological, biomedical and pharmaceutical research. The technical development of biosensors, new drugs and vaccines, and disease diagnostics heavily rely on the characterization of protein-protein interaction kinetics. The current gold standard assays for measuring protein-protein interaction are surface plasmon resonance (SPR), and bio-layer interferometry (BLI). These commercial devices are accurate but expensive, however.Here, I have developed new microfluidic techniques and models in protein-protein interaction kinetics measurement, rotational diffusion coefficient modeling, electrochemical impedance spectroscopy-based biosensors, and two-phase porous media flow models. Firstly, I applied particle diffusometry (PD) in the streptavidin-biotin binding kinetics measurement, utilizing a Y-junction microchannel. Secondly, to reduce solution volumes used in an analysis experiment, I designed a low-volume chip and coupled it with PD to measure the binding kinetics of human immunodeficiency virus p24 antibody-antigen interactions. Thirdly, considering the Brownian motion of the non-symmetric particles, I developed a new model to efficiently compute particles' rotational diffusion coefficients. Fourthly, to make economic biosensors to detect multiple biomarkers, I created a new chip, enabling hundreds of tests in a single droplet (∼ 50 μL) on one chip. Finally, to understand the liquid flow in porous media, such as nitrocellulose in lateral flow assays, I built a new two-phase porous media flow model based on the Navier-Stokes equation and compared it with experiments. These techniques and models underwent rigorous experimental and computational validation, demonstrating their effectiveness and performance.

Subject Added Entry-Topical Term  

Reagents.

Subject Added Entry-Topical Term  

Vortices.

Subject Added Entry-Topical Term  

Brownian motion.

Subject Added Entry-Topical Term  

Contact angle.

Subject Added Entry-Topical Term  

Permeability.

Subject Added Entry-Topical Term  

Geometry.

Subject Added Entry-Topical Term  

Biomedical engineering.

Subject Added Entry-Topical Term  

Fluid mechanics.

Subject Added Entry-Topical Term  

Mechanical engineering.

Index Term-Uncontrolled  

Microfluidic techniques

Index Term-Uncontrolled  

Surface plasmon resonance

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 86-06B.

Electronic Location and Access  

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Control Number  

joongbu:657544