자료유형  

 학위논문

Control Number  

0017162431

International Standard Book Number  

9798383189337

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Bassi, Tiziano Tino.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, San Diego., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

29 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-01, Section: B.

General Note  

Advisor: Toor, Navtej.

Dissertation Note  

Thesis (Ph.D.)--University of California, San Diego, 2024.

Summary, Etc.  

요약We have devised a single pot, low-cost method to add azide groups to unmodified nucleic acids without the need for enzymes or chemically modified nucleoside triphosphates. This involves reacting an azide-containing sulfinate salt with the nucleic acid, leading to replacement of C-H bonds on the nucleobase aromatic rings with C-R, where R is the azide-containing linker derived from the original sulfinate salt. With the addition of azide functional groups, the modified nucleic acid can easily be reacted with any alkyne-labeled compound of interest, including fluorescent dyes as shown in this work. This methodology enables the fluorescent labeling of a wide variety of nucleic acids, including natively folded RNAs, under mild conditions with minimal effects upon biochemical function and ribozyme catalysis. To demonstrate this, we show that a pair of labeled complementary ssDNA oligonucleotides (oligos) can hybridize to form dsDNA, even when labeled with multiple fluorophores per oligo. In addition, we also demonstrate that two different group II introns can splice when prelabeled internally with fluorophores, using our method. Broadly, this demonstrates that sulfinate modification of RNA is compatible with ribozyme function and Watson-Crick pairing, while preserving the labile backbone.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Organic chemistry.

Subject Added Entry-Topical Term  

Genetics.

Index Term-Uncontrolled  

RNA

Index Term-Uncontrolled  

Sulfinate salt

Index Term-Uncontrolled  

Fluorescent labeling

Index Term-Uncontrolled  

Oligonucleotides

Added Entry-Corporate Name  

University of California, San Diego Chemistry and Biochemistry

Host Item Entry  

Dissertations Abstracts International. 86-01B.

Electronic Location and Access  

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Control Number  

joongbu:657190