자료유형  

 학위논문

Control Number  

0017164398

International Standard Book Number  

9798346383123

Dewey Decimal Classification Number  

576.5

Main Entry-Personal Name  

Nieves-Miranda, Sharon M.

Publication, Distribution, etc. (Imprint  

[S.l.] : The Pennsylvania State University., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

225 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-05, Section: B.

General Note  

Advisor: Dudley, Edward G.

Dissertation Note  

Thesis (Ph.D.)--The Pennsylvania State University, 2024.

Summary, Etc.  

요약Escherichia coli (E. coli) O antigen serotyping serves an important role in diag-nostic, epidemiological, and public health efforts. With the new era of whole genome sequencing, the identification of novel O antigen gene clusters (O-AGCs) has surged. This study identified 32 strains harboring potential new O-AGCs. O-AGC closed se-quences were obtained via MinION nanopore sequencing, revealing O-AGCs between galF and gnd. Bioinformatic analyses indicated antimicrobial resistance genes in 50% of isolates, with some isolates classified as extraintestinal pathogenic E. coli. These findings underscore the need for updated nomenclature integration and reference col-lection expansion.O antigen serotyping has evolved from phenotypic to molecular methods, tar-geting O unit processing genes wzx/wzy or wzm/wzt. This study designed 47 PCR primer pairs organized into 10 multiplex PCR sets for the identification of 47 new O-AGCs. Primer specificity was validated against reference strains, enhancing detection accura-cy. These primers refine detection technologies, facilitating epidemiological studies and E. colisurveillance.The O antigen is believed to influence bacterial survival. This study aims to inte-grate an E. coli O-AGC sequences into a commensal E. coli DH10B K-12 strain using CRISPR-Cas9 and one-step targeted cloning. RNA-guided Cas9 cleavage targets pre-determined O-AGC sites, ligated into a cloning vector and transformed into E. coliK-12. This innovated method would aid analyses of O antigen roles in pathogenesis.

Subject Added Entry-Topical Term  

Genetic markers.

Subject Added Entry-Topical Term  

Urinary tract infections.

Subject Added Entry-Topical Term  

Drug resistance.

Subject Added Entry-Topical Term  

Scanning electron microscopy.

Subject Added Entry-Topical Term  

Zoonoses.

Subject Added Entry-Topical Term  

Analytical chemistry.

Subject Added Entry-Topical Term  

Animal diseases.

Subject Added Entry-Topical Term  

Pathology.

Subject Added Entry-Topical Term  

Pharmacology.

Added Entry-Corporate Name  

The Pennsylvania State University.

Host Item Entry  

Dissertations Abstracts International. 86-05B.

Electronic Location and Access  

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Control Number  

joongbu:657059