Material Type  

 학위논문

 

0017164029

Date and Time of Latest Transaction  

20250211152823

ISBN  

9798346567646

DDC  

574

Author  

Paul, Steffanie B.

Title/Author  

Modelling Sequence and Structure Towards Functional Protein Design.

Publish Info  

[S.l.] : Harvard University., 2024

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Material Info  

210 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-05, Section: B.

General Note  

Advisor: Marsk, Debora S.

학위논문주기  

Thesis (Ph.D.)--Harvard University, 2024.

Abstracts/Etc  

요약Millenia of evolutionary experiments have produced an extensive universe of natural macromolecular machines - proteins - that perform the variety of complex functions needed to make up a cell. In the last few decades, advances in protein engineering technologies, including the adoption of machine learning methods, have enabled us to bend and reform nature's designs towards our human needs. The advent of generative machine learning models trained on evolutionary data has enabled us to leverage nature's experiments along with years of domain knowledge to significantly move the needle on what kinds of proteins and functions we can possibly design. While these models have massive promise, there is still much to understand about i) what these models are learning, ii) how well they are learning, and iii) which models are useful for which design task. This thesis provides tools and insights to the field to shed light on these questions and thus advance our ability to engineer proteins for the functions we want.We begin with the need to identify what models perform better than others and what biological design tasks they may be useful for. Towards this, chapter 1 details our curation of the largest benchmarking dataset for generative protein models for fitness prediction, which we used to identify functional advantages for particular classes of generative models. This evaluation paradigm relies on functional measurements, which may not be available for any given protein an engineer is interested in. Thus, in chapter 2 we develop novel, statistically motivated kernel-based evaluation metrics that can be used to verify how accurately and reliably a conditional generative model has learned the distribution of the protein of interest; this provides a practitioner with helpful information about how well their model might perform for their task a priori. For highly complex functions in highly local sequence space, we argue that focused experimental data are needed to get engineering gains. In chapter 3 we discuss how machine learning models can improve the efficiency of experimental pipelines and increase our design capabilities, with a case-study on machine learning-assisted antibody optimization.

Subject Added Entry-Topical Term  

Bioinformatics.

Subject Added Entry-Topical Term  

Applied mathematics.

Subject Added Entry-Topical Term  

Bioengineering.

Subject Added Entry-Topical Term  

Evolution & development.

Index Term-Uncontrolled  

Generative AI

Index Term-Uncontrolled  

Machine learning

Index Term-Uncontrolled  

Protein engineering

Index Term-Uncontrolled  

Millenia

Index Term-Uncontrolled  

Protein design

Added Entry-Corporate Name  

Harvard University Medical Sciences

Host Item Entry  

Dissertations Abstracts International. 86-05B.

Electronic Location and Access  

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Control Number  

joongbu:656493