자료유형  

 학위논문

Control Number  

0017164697

International Standard Book Number  

9798346873167

Dewey Decimal Classification Number  

540

Main Entry-Personal Name  

Arney, Joseph Winston.

Publication, Distribution, etc. (Imprint  

[S.l.] : The University of North Carolina at Chapel Hill., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

106 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-06, Section: B.

General Note  

Advisor: Weeks, Kevin M.;Pielak, Gary.

Dissertation Note  

Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2024.

Summary, Etc.  

요약RNA molecules adopt complex structures in cells that are involved in numerous and varied biological processes. Many RNAs populate heterogeneous ensembles of structures, with states that differentially regulate biological functions. RNA structure ensembles have been challenging to detect by conventional methods for RNA structure determination, limiting our understanding of the prevalence and biological functions of RNA ensembles. To address this limitation, several recent strategies have been developed for deconvoluting RNA structure ensembles by single-molecule correlated chemical probing. In this work I summarize recent strategies for RNA ensemble deconvolution and highlight their key biological findings. I describe the validation of a structure deconvolution method developed in our lab, DANCE-MaP, as well as its application to the 7SK RNA, which revealed an RNA structure-dependent switch that globally regulates transcription. I then apply DANCE-MaP to the human SERPINA1 mRNA, revealing a hidden ensemble of structures in the early coding sequence that collectively regulates mRNA translation. Lastly, I develop an improved method for detecting RNA-ligand interactions by surface plasmon resonance. Altogether, this work demonstrates that structural heterogeneity is an intrinsic and widespread property of RNA, and that structure ensembles play critical roles in numerous biological processes. This deeper understanding of RNA structural heterogeneity might ultimately guide the development of RNA-targeting therapeutic strategies.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Biology.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Eukaryotic translation

Index Term-Uncontrolled  

RNA structure

Index Term-Uncontrolled  

Biological functions

Index Term-Uncontrolled  

Deconvolution

Added Entry-Corporate Name  

The University of North Carolina at Chapel Hill Chemistry

Host Item Entry  

Dissertations Abstracts International. 86-06B.

Electronic Location and Access  

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Control Number  

joongbu:656421