자료유형  

 학위논문

Control Number  

0017163291

International Standard Book Number  

9798384077862

Dewey Decimal Classification Number  

610

Main Entry-Personal Name  

Lopez, Santiago Caetano.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, San Francisco., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

251 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

General Note  

Includes supplementary digital materials.

General Note  

Advisor: Shipman, Seth.

Dissertation Note  

Thesis (Ph.D.)--University of California, San Francisco, 2024.

Summary, Etc.  

요약For the past 3.7 billion years, Earth has been the setting of perhaps the grandest and still ongoing genomic, evolutionary and ecological experiment: the war between bacteria and their viral parasites. Given the unfathomably large global viral and bacterial reservoirs, the former outnumbering the latter at a ratio of 10:1, it is estimated that viral transductions happen in the order of 2 x 1016 times per second. A sizeable fraction of these infections leads to prokaryotic death, and the subsequent estimated daily turnover of 15% of Earth's biomass. However, bacteria have not stood around idly: they have developed weapons of their own to fend against their viral invaders, in the form of immune systems.Over the course of evolutionary history, bacteria have developed multiple lines of defence to fend off infections, in the form of innate and adaptive immune systems. These immune systems, in turn, have been domesticated by researchers to develop novel biotechnological tools. Chapters 2 and 3 of this dissertation detail the repurposing of one of these innate immune systems, the bacterial retron, for precise genome editing in human cells, and its further engineering to enable multiple precise edits on individual genomes across the tree of life. Chapter 4 presents a study of the only known bacterial adaptive immune system, the CRISPR-Cas defence system. There, I attempt to discover novel host factors required for CRISPR adaptation, the process by which bacteria create immune memories of infection, and characterise SspA as a novel transcriptional regulator of the process.Taken together, this dissertation contends that bacterial immune systems are inseparable and cannot be properly understood in isolation of their cellular contexts, and argues for a more systems-biological understanding of their regulation and embeddedness within broader cell metabolism.

Subject Added Entry-Topical Term  

Bioengineering.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Immunology.

Index Term-Uncontrolled  

Bacterial immunity

Index Term-Uncontrolled  

Bacteriophages

Index Term-Uncontrolled  

Immune systems

Index Term-Uncontrolled  

Genome editing

Index Term-Uncontrolled  

Retron

Added Entry-Corporate Name  

University of California, San Francisco Bioengineering

Host Item Entry  

Dissertations Abstracts International. 86-03B.

Electronic Location and Access  

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Control Number  

joongbu:656080