Material Type  

 학위논문

 

0017161330

Date and Time of Latest Transaction  

20250211151340

ISBN  

9798382812526

DDC  

574

Author  

Marin, Harold Cesar.

Title/Author  

Functional Roles of Chromatin Recruitment to the Nuclear Periphery.

Publish Info  

[S.l.] : University of California, San Francisco., 2024

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Material Info  

106 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-12, Section: B.

General Note  

Advisor: Weiner, Orion.

학위논문주기  

Thesis (Ph.D.)--University of California, San Francisco, 2024.

Abstracts/Etc  

요약The eukaryotic nucleus houses deoxyribonucleic acid (DNA) along with scaffolding proteins which together form chromatin. Cells organize their chromatin as transcriptionally silent, compacted heterochromatin and active, relaxed euchromatin. Heterochromatin is enriched at the nuclear periphery in metazoans and peripheral position correlates with transcriptional repression. Here, we remove three nuclear lamins and lamin B receptor (LBR) in mouse embryonic stem cells (mESCs) and show that heterochromatin detaches from the nuclear periphery. Heterochromatic loci marked by histone H3 lysine 9 di-methylation (H3K9me2) are enriched at the nuclear periphery; however, the effect of spatial position on H3K9me2-modified genes has not been defined. Chapter 1 is a brief overview of genome organization and the major players studied in this dissertation. Chapter 2 focuses on the effect of heterochromatin spatial position on the transcription and chromatin state of H3K9me2-modified genes in naive mESCs and differentiated Epiblast-like cells (EpiLCs). Chapter 3 shows preliminary work on the effects of heterochromatin spatial position on nuclear shape, on heterochromatin-associated factors such as H3K9me3, HP1α and HP1β, on a degron system to rapidly deplete LBR, and on structure-function rescue experiments to identify regions in LBR that are important for peripheral enrichment of H3K9me2. This work shows that the nuclear periphery controls the spatial position and repressive functions H3K9me2, as well as potentially other heterochromatin marks.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Molecular biology.

Subject Added Entry-Topical Term  

Biochemistry.

Index Term-Uncontrolled  

Cell differentiation

Index Term-Uncontrolled  

H3 lysine 9 di-methylation

Index Term-Uncontrolled  

Nuclear lamina

Index Term-Uncontrolled  

Nuclear periphery

Index Term-Uncontrolled  

Deoxyribonucleic acid

Index Term-Uncontrolled  

Epiblast-like cells

Added Entry-Corporate Name  

University of California, San Francisco Biochemistry and Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 85-12B.

Electronic Location and Access  

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Control Number  

joongbu:656006