자료유형  

 학위논문

Control Number  

0017160165

International Standard Book Number  

9798382607412

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Anleu Gil, Maria Ximena.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, Davis., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

121 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-11, Section: B.

General Note  

Includes supplementary digital materials.

General Note  

Advisor: Liu, Bo.

Dissertation Note  

Thesis (Ph.D.)--University of California, Davis, 2024.

Summary, Etc.  

요약The precise spatial control of the division plane is crucial for tissue and organ patterning in plants, and the preprophase band (PPB) is a cytoskeletal structure that regulates division plane orientation in somatic cells. The PPB is primarily composed of bundled cortical microtubules (MTs) with actin and protein components and functions to organize a cortex region that becomes the future division site. The PPB-dependent cue is a positional landmark that guides centrifugal phragmoplast expansion during cytokinesis.Chapter 1 of this thesis offers a comprehensive literature review on the PPB, delving into our current understanding of the molecular mechanisms controlling its formation and function and tracing its evolutionary origins.In Chapter 2, we identify and characterize the IQ Domain (IQD) containing proteins 6/7/8 as microtubule-binding proteins associated with PPB MTs. Triple mutant plants of these IQD genes exhibit oblique cell division planes caused by disorganized PPBs that led to the misspecification of the division plane. Our studies show that robust and solid PPBs are crucial for precise division plane control and that IQD6/7/8 are necessary for properly organizing PPB MTs. Another discovery highlights the cooperation between the MT-polymerase CLIP Associated Protein (CLASP) and IQD6/7/8 in maintaining PPB MT organization and stability. Considering the conserved IQ67 domain in IQD proteins is known to bind calmodulin (CaM) in the presence of calcium (Ca2+), it raises intriguing questions about the role of Ca2+/CaM in division plane regulation.Chapter 3 focuses on the regulation of PPB formation by cyclin and cyclin-dependent kinase (CDK). We confirm that the cyclin A1;1 (CYCA1;1) and CDKA;1 localize at the PPB when expressed under native promoters, revealing an interdependence between CDKA;1 and CYCA1;1 for their PPB localization. However, the specific functions of CDKA;1 and CYCA1;1 at the PPB still need to be clarified. Our findings introduce a connection between cell cycle regulators and the mitotic cytoskeleton machinery potentially influencing PPB emergence or disassembly.In summary, these studies contribute to our understanding of molecular modules that control plant development by studying how plants control their division plane placement. Such knowledge is crucial for understanding how plants build their tissues and organs, which constitute the basis of human food systems.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Plant sciences.

Subject Added Entry-Topical Term  

Biology.

Index Term-Uncontrolled  

Cyclin-dependent kinase

Index Term-Uncontrolled  

Calcium

Index Term-Uncontrolled  

Preprophase band

Index Term-Uncontrolled  

Microtubules

Index Term-Uncontrolled  

IQ Domain

Added Entry-Corporate Name  

University of California, Davis Plant Biology

Host Item Entry  

Dissertations Abstracts International. 85-11B.

Electronic Location and Access  

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Control Number  

joongbu:655364