자료유형  

 학위논문

Control Number  

0017160347

International Standard Book Number  

9798381971675

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Herrera, Clara M.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, San Francisco., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

89 p.

General Note  

Source: Dissertations Abstracts International, Volume: 85-09, Section: B.

General Note  

Advisor: Mukherjee, Shaeri.

Dissertation Note  

Thesis (Ph.D.)--University of California, San Francisco, 2024.

Summary, Etc.  

요약Like any successful intracellular pathogen, Chlamydia trachomatis must overcome several host defenses to complete its developmental cycle. Chlamydia is thought to accomplish this through the employment of effector proteins, particularly a unique class of effectors which are inserted in the Chlamydial inclusion membrane. To test the hypothesis that these inclusion membrane proteins (Incs) target host cell proteins, our lab conducted an affinity purification-mass spectrometry screen (AP-MS) in which cells were transfected with individually tagged Incs. The predicted interaction between the Inc Tri1 and the host ubiquitin ligase TRAF7 is the subject of this work.In this dissertation, we characterize the Tri:TRAF7 complex during infection and reveal that Tri1 can disrupt native TRAF7 protein-protein interactions (PPIs, Chapter 2). Through additional analysis of this TRAF7 interactome dataset, we revealed that Tri1 can also promote novel TRAF7 PPIs (Chapter 3). Finally, we conducted RNAseq analysis to determine the role of Tri1 during infection and determined that it may play a modest role in altering signaling pathways. Overall, this work contributes to the growing literature on C. trachomatis Incs and highlights the important roles they may play during infection.

Subject Added Entry-Topical Term  

Biology.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Molecular biology.

Subject Added Entry-Topical Term  

Biochemistry.

Index Term-Uncontrolled  

Chlamydia trachomatis

Index Term-Uncontrolled  

Inclusion membrane proteins

Index Term-Uncontrolled  

Infection

Index Term-Uncontrolled  

Protein-protein interactions

Index Term-Uncontrolled  

Signaling pathways

Added Entry-Corporate Name  

University of California, San Francisco Biochemistry and Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 85-09B.

Electronic Location and Access  

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Control Number  

joongbu:654604