Material Type  

 학위논문

 

0017162589

Date and Time of Latest Transaction  

20250211152030

ISBN  

9798384024569

DDC  

540

Author  

Johnny, Christopher L.

Title/Author  

Exploration and Development of Xylopyrinde Fluorescent Tools for Spatiotemporal Imaging.

Publish Info  

[S.l.] : University of Pennsylvania., 2024

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Material Info  

239 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-02, Section: B.

General Note  

Advisor: Chenoweth, David M.

학위논문주기  

Thesis (Ph.D.)--University of Pennsylvania, 2024.

Abstracts/Etc  

요약Xylopyridine (affectionately referred to as Xylo), represents a class of small molecule fluorescent dyes known for their ability to irreversibly convert their spectral properties upon irradiation with UV light. This quality, known as photoconvertibility, belongs to a series of mechanisms we describe as photorespsonsive (PR) fluorescence. Photoactivatability and photoswitchability represent two other possible mechanisms of photoresponsive fluorescence. Photoresponsive fluorescent proteins (PRFPs) have enabled a variety of dynamic imaging applications including spatiotemporal tracking of cells, organelles, and proteins of interest. However, they are limited by their large size, poor photostability, and propensity to oligomerize. Photoresponsive small molecules (PRSMs) hold the promise of greatly improving the future of fluorescence microscopy by addressing these issues and have already enabled markedly improved performance for spatiotemporal and sub diffraction limit imaging of cells, cellular structures, and biomolecules. This thesis will describe the development and exploration of the Xylopyridine scaffold as a PRSM platform capable of improving the spatiotemporal imaging of crowded biological systems. An improved synthetic strategy for accessing the Xylo core will be described followed by rapid diversification facilitated by its ease of access. Novel photoconvertible molecules based on the Xylopyridine scaffold will then be evaluated for their spatiotemporal imaging performance. Finally, spatiotemporal imaging of cholesterol within living cells will be discussed to showcase the paradigm shifting capability of Xylopyridine fluorescent tools.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Biophysics.

Index Term-Uncontrolled  

Spatiotemporal imaging

Index Term-Uncontrolled  

Xylopyridine

Index Term-Uncontrolled  

Photorespsonsive fluorescence

Index Term-Uncontrolled  

Photoresponsive fluorescent proteins

Index Term-Uncontrolled  

Dyes

Added Entry-Corporate Name  

University of Pennsylvania Chemistry

Host Item Entry  

Dissertations Abstracts International. 86-02B.

Electronic Location and Access  

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Control Number  

joongbu:654454