자료유형  

 학위논문

Control Number  

0017163386

International Standard Book Number  

9798384023265

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Warder, Bailey Nicole.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of Pennsylvania., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

85 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-02, Section: B.

General Note  

Advisor: DiNardo, Stephen.

Dissertation Note  

Thesis (Ph.D.)--University of Pennsylvania, 2024.

Summary, Etc.  

요약Stem cells often rely on signals from a niche, which in many tissues adopts a precise morphology. What remains elusive is how niches are formed, and how niche morphology impacts its function. To address this, we leverage the Drosophila gonadal niche, which affords genetic tractability and live-imaging. We have previously shown mechanisms dictating niche cell migration to their appropriate position within the gonad, and the resultant consequences on niche function. Here, utilizing a combination of fixed and live-imaging approaches, we show that once positioned, niche cells robustly polarize filamentous actin (F-actin) and Non-muscle Myosin II (MyoII) towards neighboring germ cells. We utilize laser severing of the actomyosin network to reveal polarized actomyosin tension along the niche periphery. By manipulating actomyosin contractility (AMC) with a combination of pharmacological and transgenic approaches, we find that this tension generates a highly reproducible smoothened niche contour. Without contractility, niches are misshapen and exhibit functional defects in their ability to limit self-renewal signals and regulate germline stem cell divisions. We additionally examined the upstream mechanisms that polarize MyoII along the niche periphery. By ablating germ cells and inhibiting their division, we show that germ cells aid in polarizing MyoII within niche cells, and that extrinsic input is required for niche morphogenesis and function. Our work reveals a feedback mechanism where stem cells shape the niche that guides their behavior.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Developmental biology.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Actomyosin contractility

Index Term-Uncontrolled  

Drosophila

Index Term-Uncontrolled  

Morphogenesis

Index Term-Uncontrolled  

Niche

Index Term-Uncontrolled  

Stem cell

Added Entry-Corporate Name  

University of Pennsylvania Cell and Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 86-02B.

Electronic Location and Access  

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Control Number  

joongbu:654305