자료유형  

 학위논문

Control Number  

0017163429

International Standard Book Number  

9798896077237

Dewey Decimal Classification Number  

540

Main Entry-Personal Name  

Warner, Elliot A.

Publication, Distribution, etc. (Imprint  

[S.l.] : The Scripps Research Institute., 2024

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2024

Physical Description  

92 p.

General Note  

Source: Dissertations Abstracts International, Volume: 86-04, Section: B.

General Note  

Advisor: Schultz, Peter G.

Dissertation Note  

Thesis (Ph.D.)--The Scripps Research Institute, 2024.

Summary, Etc.  

요약The ability to incorporate novel chemistries into proteins with non-canonical amino acids (ncAAs) allows researchers to study and build proteins in ways unconstrained by the activities available within the standard biologic code. In this thesis, we made efforts to build tetrazine-containing ncAAs tools that can phototrigger microsecond protein folding events.While protein folding is a fundamental biological process, most study is limited to physical theory and computational modeling. Actual experimental observation is hampered by a lack of general tools to reliably study folding processes that occur on nanosecond-to-microsecond timescales. Current experimental approaches are experimentally arduous, often must be performed in the presence of chaotropic agents that can alter folding pathways and only capture a small portion of the complexity of possible folding pathways.Here, we describe efforts towards adapting tetrazine-containing ncAAs into general, site-specific phototriggers of protein folding. Specifically, we identify residue sites in model folding proteins that have differential stability between tetrazine ncAAs and their photodecomposition products. We show that we can induce tetrazine photolysis in proteins while minimizing protein damage. Lastly, we build out a labeling and time-resolved spectroscopic monitoring strategy that should allow the phototriggering and monitoring of protein folding at single digit microsecond timescales.

Subject Added Entry-Topical Term  

Chemistry.

Subject Added Entry-Topical Term  

Molecular biology.

Subject Added Entry-Topical Term  

Biochemistry.

Index Term-Uncontrolled  

Protein

Index Term-Uncontrolled  

Phototrigger

Index Term-Uncontrolled  

Tetrazine

Index Term-Uncontrolled  

Microsecond timescales

Added Entry-Corporate Name  

The Scripps Research Institute Chemical Biology

Host Item Entry  

Dissertations Abstracts International. 86-04B.

Electronic Location and Access  

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Control Number  

joongbu:653838