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Responsive Dual Nanoparticle 19F Magnetic Resonance Molecular Imaging Probes.
Responsive Dual Nanoparticle 19F Magnetic Resonance Molecular Imaging Probes.
상세정보
- 자료유형
- 학위논문
- Control Number
- 0017164976
- International Standard Book Number
- 9798384441250
- Dewey Decimal Classification Number
- 616
- Main Entry-Personal Name
- Cooke, Daniel J.
- Publication, Distribution, etc. (Imprint
- [S.l.] : The University of Texas at Austin., 2024
- Publication, Distribution, etc. (Imprint
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- Physical Description
- 169 p.
- General Note
- Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
- General Note
- Advisor: Que, Emily L.
- Dissertation Note
- Thesis (Ph.D.)--The University of Texas at Austin, 2024.
- Summary, Etc.
- 요약Noninvasive molecular imaging is a powerful tool for the diagnosis of disease. Magnetic resonance imaging (MRI) is an excellent candidate for molecular imaging due to a lack of ionizing radiation, high spatial resolution and unlimited penetration depth. 19F MRI benefits from a very low biological background signal and can provide complementary information to the more commonly used 1H MRI. This dissertation reports the study of responsive dual nanoparticle molecular imaging probes for 19F MRI. We use perfluorocarbon-encapsulated mesoporous silica nanoparticles (19F-MSNs) bound to a magnetic quencher nanoparticle to modulate the 19F MR signal. The nature of the linkage between the nanoparticles allows for signal "turn-on" in response to an external stimulus, facilitating specific detection of biological analytes or environments.We have developed a dual nanoparticle conjugate probe using superparamagnetic iron oxide nanoparticles (SPIONs) to quench the 19F MR signal of 19F-MSNs. Using DNA aptamers as linkers, a robust 19F MRI response is observed in live mice in the presence of thrombin. The use of DNA aptamers allows for versatility of analyte sensing. Improvements can be made to this probe using two main strategies: 1) Incorporating T1 shortening agents to increase the conspicuity of the probe; 2) Varying the size of the SPION and its distance to 19FMSNs. We propose that paramagnetic MnO nanoparticles incorporated into the perfluorocarbon phase of 19F-MSNs can enhance the 19F T1 relaxation time. The solubilization of MnO in perfluorocarbons necessitates ligand exchange with fluorinated ligands. The size of SPIONs affects their quenching strength and can be easily tuned by changing the synthetic parameters. The distance between SPIONs and 19F-MSNs is tunable by varying the length of the DNA linkers. We discuss methods to enhance the sensitivity of DNC by varying these parameters.Lastly, we are adapting the DNC platform to be a theranostic probe by replacing SPIONs with superparamagnetic FePt nanoparticles. We demonstrate that FePt nanoparticles are cytotoxic due to the release of Fe2+ and Pt2+ at low pH upon cellular uptake. FePt nanoparticles are bound to 19F-MSNs by a pHsensitive linker such that FePt is released from 19F-MSNs in the low pH extracellular tumor microenvironment.
- Subject Added Entry-Topical Term
- Medical imaging.
- Subject Added Entry-Topical Term
- Molecular biology.
- Subject Added Entry-Topical Term
- Nanoscience.
- Index Term-Uncontrolled
- Noninvasive molecular imaging
- Index Term-Uncontrolled
- Magnetic resonance imaging
- Index Term-Uncontrolled
- Nanoparticles
- Index Term-Uncontrolled
- Biological analytes
- Added Entry-Corporate Name
- The University of Texas at Austin Chemistry
- Host Item Entry
- Dissertations Abstracts International. 86-04B.
- Electronic Location and Access
- 로그인을 한후 보실 수 있는 자료입니다.
- Control Number
- joongbu:653646
MARC
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■035 ▼a(MiAaPQ)123vireo24688Cooke
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a616
■1001 ▼aCooke, Daniel J.
■24510▼aResponsive Dual Nanoparticle 19F Magnetic Resonance Molecular Imaging Probes.
■260 ▼a[S.l.]▼bThe University of Texas at Austin. ▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a169 p.
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-04, Section: B.
■500 ▼aAdvisor: Que, Emily L.
■5021 ▼aThesis (Ph.D.)--The University of Texas at Austin, 2024.
■520 ▼aNoninvasive molecular imaging is a powerful tool for the diagnosis of disease. Magnetic resonance imaging (MRI) is an excellent candidate for molecular imaging due to a lack of ionizing radiation, high spatial resolution and unlimited penetration depth. 19F MRI benefits from a very low biological background signal and can provide complementary information to the more commonly used 1H MRI. This dissertation reports the study of responsive dual nanoparticle molecular imaging probes for 19F MRI. We use perfluorocarbon-encapsulated mesoporous silica nanoparticles (19F-MSNs) bound to a magnetic quencher nanoparticle to modulate the 19F MR signal. The nature of the linkage between the nanoparticles allows for signal "turn-on" in response to an external stimulus, facilitating specific detection of biological analytes or environments.We have developed a dual nanoparticle conjugate probe using superparamagnetic iron oxide nanoparticles (SPIONs) to quench the 19F MR signal of 19F-MSNs. Using DNA aptamers as linkers, a robust 19F MRI response is observed in live mice in the presence of thrombin. The use of DNA aptamers allows for versatility of analyte sensing. Improvements can be made to this probe using two main strategies: 1) Incorporating T1 shortening agents to increase the conspicuity of the probe; 2) Varying the size of the SPION and its distance to 19FMSNs. We propose that paramagnetic MnO nanoparticles incorporated into the perfluorocarbon phase of 19F-MSNs can enhance the 19F T1 relaxation time. The solubilization of MnO in perfluorocarbons necessitates ligand exchange with fluorinated ligands. The size of SPIONs affects their quenching strength and can be easily tuned by changing the synthetic parameters. The distance between SPIONs and 19F-MSNs is tunable by varying the length of the DNA linkers. We discuss methods to enhance the sensitivity of DNC by varying these parameters.Lastly, we are adapting the DNC platform to be a theranostic probe by replacing SPIONs with superparamagnetic FePt nanoparticles. We demonstrate that FePt nanoparticles are cytotoxic due to the release of Fe2+ and Pt2+ at low pH upon cellular uptake. FePt nanoparticles are bound to 19F-MSNs by a pHsensitive linker such that FePt is released from 19F-MSNs in the low pH extracellular tumor microenvironment.
■590 ▼aSchool code: 0227.
■650 4▼aMedical imaging.
■650 4▼aMolecular biology.
■650 4▼aNanoscience.
■653 ▼aNoninvasive molecular imaging
■653 ▼aMagnetic resonance imaging
■653 ▼aNanoparticles
■653 ▼aBiological analytes
■690 ▼a0565
■690 ▼a0574
■690 ▼a0307
■71020▼aThe University of Texas at Austin▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g86-04B.
■790 ▼a0227
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17164976▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.