자료유형  

 학위논문

Control Number  

0016934540

International Standard Book Number  

9798380485890

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Jones, Taylor.

Publication, Distribution, etc. (Imprint  

[S.l.] : Stanford University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(183 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

General Note  

Advisor: Cui, Bianxiao.

Dissertation Note  

Thesis (Ph.D.)--Stanford University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Nanoscale membrane curvature is understood to play an active role in essential cellular processes such as endocytosis, exocytosis, and actin dynamics. Few methods, however, can precisely manipulate membrane curvature in live cells. Making use of BAR domain proteins, a well-studied superfamily of membraneremodeling proteins, and the improved Light-Inducible Dimer (iLID) system, we developed a new method of generating nanoscale membrane curvature in live cells that is controllable, reversible, and capable of precise spatial and temporal manipulation. As proof of concept, we first engineered two optogenetic systems, opto-FBAR and opto-IBAR, that allow light-inducible formation of inward and outward membrane curvature, respectively. We then expanded upon this approach by engineering other BAR domain superfamily members to be light-inducible, including members of the FCHO, PACSIN, Amphiphysin, and NOSTRIN subfamilies. These systems present a novel approach for light-inducible manipulation of nanoscale membrane curvature in live cells.

Subject Added Entry-Topical Term  

Membranes.

Subject Added Entry-Topical Term  

Plasmids.

Subject Added Entry-Topical Term  

Signal transduction.

Subject Added Entry-Topical Term  

Plasma.

Subject Added Entry-Topical Term  

Cell adhesion & migration.

Subject Added Entry-Topical Term  

Sensors.

Subject Added Entry-Topical Term  

Microscopy.

Subject Added Entry-Topical Term  

Engineering.

Subject Added Entry-Topical Term  

E coli.

Subject Added Entry-Topical Term  

Lipids.

Subject Added Entry-Topical Term  

Deformation.

Subject Added Entry-Topical Term  

Cell culture.

Subject Added Entry-Topical Term  

Cellular biology.

Added Entry-Corporate Name  

Stanford University.

Host Item Entry  

Dissertations Abstracts International. 85-04B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:643571