자료유형  

 학위논문

Control Number  

0016935189

International Standard Book Number  

9798380713030

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Laosuntisuk, Kanjana.

Publication, Distribution, etc. (Imprint  

[S.l.] : North Carolina State University., 2022

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Physical Description  

1 online resource(286 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-05, Section: B.

General Note  

Advisor: Doherty, Colleen.

Dissertation Note  

Thesis (Ph.D.)--North Carolina State University, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Plants utilize the circadian clock to coordinate biological processes in response to daily and seasonal changes to maximize fitness. Intense global warming generates unusual patterns of weather and climate including shorter winter and longer summer and increases both daytime and nighttime temperatures. This work focuses on the importance of the time of day in analyzing the transcriptional responses under temperature stress. First, we investigated transcriptional changes in response to chilling stress at dawn and dusk in sorghum using 3' RNA-Sequencing (3' RNASeq). Low temperatures in the early season hinder heat stress escape. Heat escape is a strategy where the growing season is shifted earlier to avoid heat stress during flowering time, a heatsensitive period that is important for determining yields. To improve the accuracy of RNA-Seq analysis, we tested the RNA-Seq normalization methods using synthetic RNA spike-in controls and found that an accumulation of transcripts increased at night, resulting in increased detection of nighttime-upregulated genes. Then, we determined the interaction effect of the time of day and chilling stress and found that the time of day gates a differential expression under chilling stress. We identified the candidate transcription factors regulating genes that increased in expression under chilling stress only at night, suggesting that the candidates can integrate both time and temperature information. Additionally, we characterized the circadian oscillation in Arabidopsis callus and cell suspension culture that showed defects in the oscillation under the continuous light and temperature cycles. We found that ELF3 can partially recover these defects. We further reviewed the role of ELF3 in the literature as a temperature sensor and the connection between the circadian clock and heat stress-responsive pathway. Lastly, we identified the connection between N-linked glycosylation and the circadian clock by characterizing plant Asparagine-linked Glycosylation 10 (ALG10). In conclusion, this work highlights the importance of the time of day and the circadian clock in gene expression at the transcriptional level in intact plants or cell cultures under normal conditions or temperature stress. Including time as a factor in the experimental design provides novel and valuable information to better understand plant molecular biology.

Subject Added Entry-Topical Term  

Signal transduction.

Subject Added Entry-Topical Term  

RNA polymerase.

Subject Added Entry-Topical Term  

Gene expression.

Subject Added Entry-Topical Term  

Agricultural production.

Subject Added Entry-Topical Term  

Flowers & plants.

Subject Added Entry-Topical Term  

Domestication.

Subject Added Entry-Topical Term  

Algae.

Subject Added Entry-Topical Term  

Seeds.

Subject Added Entry-Topical Term  

Abiotic stress.

Subject Added Entry-Topical Term  

Sorghum.

Subject Added Entry-Topical Term  

Heat.

Subject Added Entry-Topical Term  

Genomes.

Subject Added Entry-Topical Term  

Circadian rhythm.

Subject Added Entry-Topical Term  

Summer.

Subject Added Entry-Topical Term  

Radiation.

Subject Added Entry-Topical Term  

Transcription factors.

Subject Added Entry-Topical Term  

Plant reproduction.

Subject Added Entry-Topical Term  

Agriculture.

Subject Added Entry-Topical Term  

Agronomy.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Bioinformatics.

Subject Added Entry-Topical Term  

Botany.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Neurosciences.

Subject Added Entry-Topical Term  

Plant sciences.

Added Entry-Corporate Name  

North Carolina State University.

Host Item Entry  

Dissertations Abstracts International. 85-05B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:642918