Material Type  

 학위논문

 

0016934245

Date and Time of Latest Transaction  

20240214101546

ISBN  

9798380112970

DDC  

616.079

Author  

Hunter, Joseph.

Title/Author  

The Expression of Complex Proteins in Plant Systems for Industrial and Biomedical Applications - [electronic resource]

Publish Info  

[S.l.] : Arizona State University., 2023

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Material Info  

1 online resource(111 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-02, Section: B.

General Note  

Advisor: Mason, Hugh.

학위논문주기  

Thesis (Ph.D.)--Arizona State University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Abstracts/Etc  

요약The expression of complex proteins was studied in multiple plant systems. Recombinant spider silk, which could be utilized for biomedical applications such as coatings or doped into silk fibers, was successfully expressed in Nicotiana. benthamiana wild type and GnGn glycoengineered transgenic plants and purified from endogenous plant proteins which could be utilized for biomedical applications such as coatings or doped into silk fibers. However, the purification process requires further optimization to result in commercialized production of recombinant spider silk. Green fluorescent protein and Norovirus virus-like particles were expressed in multiple plant systems including alfalfa, beets, lettuce, and spinach, in addition to N. benthamiana, to determine the ability of these plant expression systems to produce vaccine candidates for edible vaccine applications in the agricultural sector as well as low-to-middle income countries. It was determined that alfalfa, beets, and lettuce are potential high production expression systems for edible vaccines however they require further optimization to be commercialized. Lastly, novel virus-like particles and antigen presenting nanoparticles based on the bacteriophage AP205 coat protein and norovirus capsid proteins fused to human papillomavirus L2 protein segments (S and P) were expressed in N. benthamiana and utilized to vaccinate mice against the L2 capsid protein (aa14-38x2 and aa14-122) of Human Papillomavirus 16 to study a potential boosting effect of the Recombinant Immune Complex vaccine platform upon prime-boost dosing with the virus-like particle being the prime and the Recombinant Immune Complex being the boost in this vaccine schema.

Subject Added Entry-Topical Term  

Immunology.

Subject Added Entry-Topical Term  

Virology.

Subject Added Entry-Topical Term  

Biology.

Index Term-Uncontrolled  

Plant systems

Index Term-Uncontrolled  

Proteins

Index Term-Uncontrolled  

Biomedical applications

Index Term-Uncontrolled  

Edible vaccine

Index Term-Uncontrolled  

Papillomavirus

Added Entry-Corporate Name  

Arizona State University Molecular and Cellular Biology

Host Item Entry  

Dissertations Abstracts International. 85-02B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

로그인을 한후 보실 수 있는 자료입니다.

소장사항  

202402 2024

Control Number  

joongbu:642814