자료유형  

 학위논문

Control Number  

0016935168

International Standard Book Number  

9798380072298

Dewey Decimal Classification Number  

636

Main Entry-Personal Name  

Westbrook, Lindsay A.

Publication, Distribution, etc. (Imprint  

[S.l.] : Texas A&M University., 2022

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Physical Description  

1 online resource(93 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-02, Section: B.

General Note  

Advisor: Smith, Stephen B.

Dissertation Note  

Thesis (Ph.D.)--Texas A&M University, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Two experiments were conducted to demonstrate functional G-coupled protein receptor 43 (GPR43) and GPR120 activity in bovine intramuscular (i.m.) and subcutaneous (s.c.) adipose tissues. We hypothesized that media volatile fatty acids and long- chain fatty acids (LCFA) would affect cAMP concentration, glycerol concentration, and free fatty acid (FFA) concentration differently in i.m. and s.c. adipose tissues taken from steers at 20, 22, 24 mo of age. Fresh s.c. and i.m. adipose tissue from the 5th to 8th longissimus thoracic rib muscle section of Angus crossbred steers was transferred immediately to 6-well culture plates containing 3 mL of KHB/Hepes/5 mM glucose. Samples were preincubated with 0.5 mM theophylline plus 10 μM forskolin for 30 min, after which increasing concentrations of acetate or propionate (0, 1, 5, and 10 mM) in the absence or the presence of 100 μM oleic acid (18:1 n-9) or 100 μM palmitic acid (16:0) were added to the incubation media. In s.c. adipose tissue, cAMP production decreased in the presence of LCFA (P  0.0001), suggesting that LCFA may induce lipid filling in s.c. adipose tissue, which is consistent with GPR120 receptor activity. At 22 mo of age, the addition of VFAs in the presence of LCFA effectively decreased cAMP production (P = 0.03), indicating increased lipid accumulation at 22 mo of age (P = 0.03). Free fatty acid concentration in the absence of LCFA was greater in i.m. adipose tissue at 22 mo of age (P  0.0001). The relative expression of GPR120 was greater in s.c adipose tissue than i.m. adipose tissue (P = 0.05). These results provide evidence for functional GPR43 receptors in i.m. adipose tissue and GPR120 receptors in s.c. adipose tissue, both of which suppress lipolysis.

Subject Added Entry-Topical Term  

Animal sciences.

Subject Added Entry-Topical Term  

Physiology.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Biochemistry.

Index Term-Uncontrolled  

Acetate

Index Term-Uncontrolled  

Adipose tissue

Index Term-Uncontrolled  

Bovine

Index Term-Uncontrolled  

Oleic acid

Index Term-Uncontrolled  

Palmitic acid

Index Term-Uncontrolled  

GPR receptors

Added Entry-Corporate Name  

Texas A&M University Animal Science

Host Item Entry  

Dissertations Abstracts International. 85-02B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

로그인을 한후 보실 수 있는 자료입니다.

Control Number  

joongbu:642703