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Development and Characterization of Selective and Differential Culture Medium for the Poultry Transmitted Pathogen, Escherichia albertii- [electronic resource]
Development and Characterization of Selective and Differential Culture Medium for the Poultry Transmitted Pathogen, Escherichia albertii- [electronic resource]
상세정보
- 자료유형
- 학위논문
- Control Number
- 0016935859
- International Standard Book Number
- 9798380829052
- Dewey Decimal Classification Number
- 641
- Main Entry-Personal Name
- Annor, Samuel Dominic.
- Publication, Distribution, etc. (Imprint
- [S.l.] : Texas A&M University., 2023
- Publication, Distribution, etc. (Imprint
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- Physical Description
- 1 online resource(67 p.)
- General Note
- Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
- General Note
- Advisor: Taylor, Thomas M.
- Dissertation Note
- Thesis (Ph.D.)--Texas A&M University, 2023.
- Restrictions on Access Note
- This item must not be sold to any third party vendors.
- Summary, Etc.
- 요약Escherichia albertii is an emerging foodborne enteropathogen. It was first isolated in 1991 from a 9-month-old febrile Bangladeshi girl with diarrhea, and retrospectively found to be the causative agent in a human foodborne disease outbreak occurring at the end of May 2011 in Kumamoto, Japan. Previous phenotypic, biochemical, genetic, and microbiological studies have misidentified this under-researched bacterial foodborne pathogen, and this has impeded accurate assessment by food safety scientists and epidemiologists of the burden borne by this organism to U.S. and/or global food safety. Despite enjoying increased attention paid to its pathogenesis, global dissemination, and antimicrobial resistance capacity, this foodborne pathogen remains difficult to identify from human foods. The primary objective of this study was to develop and test a selective and differential plating medium for the isolation of E. albertii from enteric pathogens commonly transmitted via fresh poultry meat, namely E. coli and Salmonella enterica. MacConkey agar was supplemented with α-D-+-melibiose and the lactose analogue X-gal and utilized to differentially enumerate E. albertii, Salmonella, and E. coli from inoculated ground chicken meat. The medium, MXgMac agar, differentiated the inoculated pathogens with a greater degree of efficiency than did the previously developed E. albertii-selective medium xylose-rhamnose-melibiose (XRM) MacConkey agar, based on differential usage of the lactose analogue and melibiose. Chicken-derived feces and litter samples were subsequently tested using the medium and found not to contain E. albertii by 16S rRNA gene amplification. In conclusion, MXgMac agar facilitates improved differential recovery of E. albertii and other enteric pathogens from poultry meat versus other E. albertii selective/differential media. It is hoped that this novel cultural medium could be utilized to explore further this pathogen's virulence mechanisms and potentials, nutrient utilization, stress tolerance capacity, and their regulation.
- Subject Added Entry-Topical Term
- Food science.
- Subject Added Entry-Topical Term
- Microbiology.
- Subject Added Entry-Topical Term
- Pathology.
- Index Term-Uncontrolled
- Escherichia albertii
- Index Term-Uncontrolled
- Poultry safety
- Index Term-Uncontrolled
- Food safety
- Index Term-Uncontrolled
- Foodborne disease
- Index Term-Uncontrolled
- E. coli
- Index Term-Uncontrolled
- Salmonella
- Added Entry-Corporate Name
- Texas A&M University Food Science and Technology
- Host Item Entry
- Dissertations Abstracts International. 85-05B.
- Host Item Entry
- Dissertation Abstract International
- Electronic Location and Access
- 로그인을 한후 보실 수 있는 자료입니다.
- Control Number
- joongbu:642543
MARC
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■007cr#unu||||||||
■020 ▼a9798380829052
■035 ▼a(MiAaPQ)AAI30873046
■035 ▼a(MiAaPQ)0803vireo27741Annor
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a641
■1001 ▼aAnnor, Samuel Dominic.
■24510▼aDevelopment and Characterization of Selective and Differential Culture Medium for the Poultry Transmitted Pathogen, Escherichia albertii▼h[electronic resource]
■260 ▼a[S.l.]▼bTexas A&M University. ▼c2023
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2023
■300 ▼a1 online resource(67 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-05, Section: B.
■500 ▼aAdvisor: Taylor, Thomas M.
■5021 ▼aThesis (Ph.D.)--Texas A&M University, 2023.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aEscherichia albertii is an emerging foodborne enteropathogen. It was first isolated in 1991 from a 9-month-old febrile Bangladeshi girl with diarrhea, and retrospectively found to be the causative agent in a human foodborne disease outbreak occurring at the end of May 2011 in Kumamoto, Japan. Previous phenotypic, biochemical, genetic, and microbiological studies have misidentified this under-researched bacterial foodborne pathogen, and this has impeded accurate assessment by food safety scientists and epidemiologists of the burden borne by this organism to U.S. and/or global food safety. Despite enjoying increased attention paid to its pathogenesis, global dissemination, and antimicrobial resistance capacity, this foodborne pathogen remains difficult to identify from human foods. The primary objective of this study was to develop and test a selective and differential plating medium for the isolation of E. albertii from enteric pathogens commonly transmitted via fresh poultry meat, namely E. coli and Salmonella enterica. MacConkey agar was supplemented with α-D-+-melibiose and the lactose analogue X-gal and utilized to differentially enumerate E. albertii, Salmonella, and E. coli from inoculated ground chicken meat. The medium, MXgMac agar, differentiated the inoculated pathogens with a greater degree of efficiency than did the previously developed E. albertii-selective medium xylose-rhamnose-melibiose (XRM) MacConkey agar, based on differential usage of the lactose analogue and melibiose. Chicken-derived feces and litter samples were subsequently tested using the medium and found not to contain E. albertii by 16S rRNA gene amplification. In conclusion, MXgMac agar facilitates improved differential recovery of E. albertii and other enteric pathogens from poultry meat versus other E. albertii selective/differential media. It is hoped that this novel cultural medium could be utilized to explore further this pathogen's virulence mechanisms and potentials, nutrient utilization, stress tolerance capacity, and their regulation.
■590 ▼aSchool code: 0803.
■650 4▼aFood science.
■650 4▼aMicrobiology.
■650 4▼aPathology.
■653 ▼aEscherichia albertii
■653 ▼aPoultry safety
■653 ▼aFood safety
■653 ▼aFoodborne disease
■653 ▼aE. coli
■653 ▼aSalmonella
■690 ▼a0359
■690 ▼a0410
■690 ▼a0571
■71020▼aTexas A&M University▼bFood Science and Technology.
■7730 ▼tDissertations Abstracts International▼g85-05B.
■773 ▼tDissertation Abstract International
■790 ▼a0803
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16935859▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
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