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Development and Characterization of Selective and Differential Culture Medium for the Poultry Transmitted Pathogen, Escherichia albertii- [electronic resource]
Development and Characterization of Selective and Differential Culture Medium for the Poultry Transmitted Pathogen, Escherichia albertii- [electronic resource]

상세정보

자료유형  
 학위논문
Control Number  
0016935859
International Standard Book Number  
9798380829052
Dewey Decimal Classification Number  
641
Main Entry-Personal Name  
Annor, Samuel Dominic.
Publication, Distribution, etc. (Imprint  
[S.l.] : Texas A&M University., 2023
Publication, Distribution, etc. (Imprint  
Ann Arbor : ProQuest Dissertations & Theses, 2023
Physical Description  
1 online resource(67 p.)
General Note  
Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
General Note  
Advisor: Taylor, Thomas M.
Dissertation Note  
Thesis (Ph.D.)--Texas A&M University, 2023.
Restrictions on Access Note  
This item must not be sold to any third party vendors.
Summary, Etc.  
요약Escherichia albertii is an emerging foodborne enteropathogen. It was first isolated in 1991 from a 9-month-old febrile Bangladeshi girl with diarrhea, and retrospectively found to be the causative agent in a human foodborne disease outbreak occurring at the end of May 2011 in Kumamoto, Japan. Previous phenotypic, biochemical, genetic, and microbiological studies have misidentified this under-researched bacterial foodborne pathogen, and this has impeded accurate assessment by food safety scientists and epidemiologists of the burden borne by this organism to U.S. and/or global food safety. Despite enjoying increased attention paid to its pathogenesis, global dissemination, and antimicrobial resistance capacity, this foodborne pathogen remains difficult to identify from human foods. The primary objective of this study was to develop and test a selective and differential plating medium for the isolation of E. albertii from enteric pathogens commonly transmitted via fresh poultry meat, namely E. coli and Salmonella enterica. MacConkey agar was supplemented with α-D-+-melibiose and the lactose analogue X-gal and utilized to differentially enumerate E. albertii, Salmonella, and E. coli from inoculated ground chicken meat. The medium, MXgMac agar, differentiated the inoculated pathogens with a greater degree of efficiency than did the previously developed E. albertii-selective medium xylose-rhamnose-melibiose (XRM) MacConkey agar, based on differential usage of the lactose analogue and melibiose. Chicken-derived feces and litter samples were subsequently tested using the medium and found not to contain E. albertii by 16S rRNA gene amplification. In conclusion, MXgMac agar facilitates improved differential recovery of E. albertii and other enteric pathogens from poultry meat versus other E. albertii selective/differential media. It is hoped that this novel cultural medium could be utilized to explore further this pathogen's virulence mechanisms and potentials, nutrient utilization, stress tolerance capacity, and their regulation.
Subject Added Entry-Topical Term  
Food science.
Subject Added Entry-Topical Term  
Microbiology.
Subject Added Entry-Topical Term  
Pathology.
Index Term-Uncontrolled  
Escherichia albertii
Index Term-Uncontrolled  
Poultry safety
Index Term-Uncontrolled  
Food safety
Index Term-Uncontrolled  
Foodborne disease
Index Term-Uncontrolled  
E. coli
Index Term-Uncontrolled  
Salmonella
Added Entry-Corporate Name  
Texas A&M University Food Science and Technology
Host Item Entry  
Dissertations Abstracts International. 85-05B.
Host Item Entry  
Dissertation Abstract International
Electronic Location and Access  
로그인을 한후 보실 수 있는 자료입니다.
Control Number  
joongbu:642543

MARC

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■020    ▼a9798380829052
■035    ▼a(MiAaPQ)AAI30873046
■035    ▼a(MiAaPQ)0803vireo27741Annor
■040    ▼aMiAaPQ▼cMiAaPQ
■0820  ▼a641
■1001  ▼aAnnor,  Samuel  Dominic.
■24510▼aDevelopment  and  Characterization  of  Selective  and  Differential  Culture  Medium  for  the  Poultry  Transmitted  Pathogen,  Escherichia  albertii▼h[electronic  resource]
■260    ▼a[S.l.]▼bTexas  A&M  University.  ▼c2023
■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2023
■300    ▼a1  online  resource(67  p.)
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  85-05,  Section:  B.
■500    ▼aAdvisor:  Taylor,  Thomas  M.
■5021  ▼aThesis  (Ph.D.)--Texas  A&M  University,  2023.
■506    ▼aThis  item  must  not  be  sold  to  any  third  party  vendors.
■520    ▼aEscherichia  albertii  is  an  emerging  foodborne  enteropathogen.  It  was  first  isolated  in  1991  from  a  9-month-old  febrile  Bangladeshi  girl  with  diarrhea,  and  retrospectively  found  to  be  the  causative  agent  in  a  human  foodborne  disease  outbreak  occurring  at  the  end  of  May  2011  in  Kumamoto,  Japan.  Previous  phenotypic,  biochemical,  genetic,  and  microbiological  studies  have  misidentified  this  under-researched  bacterial  foodborne  pathogen,  and  this  has  impeded  accurate  assessment  by  food  safety  scientists  and  epidemiologists  of  the  burden  borne  by  this  organism  to  U.S.  and/or  global  food  safety.  Despite  enjoying  increased  attention  paid  to  its  pathogenesis,  global  dissemination,  and  antimicrobial  resistance  capacity,  this  foodborne  pathogen  remains  difficult  to  identify  from  human  foods.  The  primary  objective  of  this  study  was  to  develop  and  test  a  selective  and  differential  plating  medium  for  the  isolation  of  E.  albertii  from  enteric  pathogens  commonly  transmitted  via  fresh  poultry  meat,  namely  E.  coli  and  Salmonella  enterica.  MacConkey  agar  was  supplemented  with  α-D-+-melibiose  and  the  lactose  analogue  X-gal  and  utilized  to  differentially  enumerate  E.  albertii,  Salmonella,  and  E.  coli  from  inoculated  ground  chicken  meat.  The  medium,  MXgMac  agar,  differentiated  the  inoculated  pathogens  with  a  greater  degree  of  efficiency  than  did  the  previously  developed  E.  albertii-selective  medium  xylose-rhamnose-melibiose  (XRM)  MacConkey  agar,  based  on  differential  usage  of  the  lactose  analogue  and  melibiose.  Chicken-derived  feces  and  litter  samples  were  subsequently  tested  using  the  medium  and  found  not  to  contain  E.  albertii  by  16S  rRNA  gene  amplification.  In  conclusion,  MXgMac  agar  facilitates  improved  differential  recovery  of  E.  albertii  and  other  enteric  pathogens  from  poultry  meat  versus  other  E.  albertii  selective/differential  media.  It  is  hoped  that  this  novel  cultural  medium  could  be  utilized  to  explore  further  this  pathogen's virulence  mechanisms  and  potentials,  nutrient  utilization,  stress  tolerance  capacity,  and  their  regulation.
■590    ▼aSchool  code:  0803.
■650  4▼aFood  science.
■650  4▼aMicrobiology.
■650  4▼aPathology.
■653    ▼aEscherichia  albertii
■653    ▼aPoultry  safety
■653    ▼aFood  safety
■653    ▼aFoodborne  disease
■653    ▼aE.  coli
■653    ▼aSalmonella
■690    ▼a0359
■690    ▼a0410
■690    ▼a0571
■71020▼aTexas  A&M  University▼bFood  Science  and  Technology.
■7730  ▼tDissertations  Abstracts  International▼g85-05B.
■773    ▼tDissertation  Abstract  International
■790    ▼a0803
■791    ▼aPh.D.
■792    ▼a2023
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16935859▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.
■980    ▼a202402▼f2024

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