자료유형  

 학위논문

Control Number  

0016934477

International Standard Book Number  

9798380481878

Dewey Decimal Classification Number  

610

Main Entry-Personal Name  

Kobak, Kayla C.

Publication, Distribution, etc. (Imprint  

[S.l.] : Stanford University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(67 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-04, Section: A.

General Note  

Advisor: Baker, Julie.

Dissertation Note  

Thesis (Ph.D.)--Stanford University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약The placenta, a vital organ for fetal development and species propagation, plays a crucial role in sustaining the fetus by providing nutrients, oxygen, and hormonal support. Despite extensive morphological descriptions, the molecular organization and characterization of cell types within the placenta remain limited. Recent advancements in single-cell RNA sequencing technologies have revealed various trophoblast and fetal cell subtypes, but the open-chromatin regulatory landscape has yet to be described. This dissertation aims to address this knowledge gap by employing a multi-dimensional approach, encompassing single-cell transcriptomics, epigenomics, and copy number variation (CNV) analysis. The study explores placental dynamics across the first trimester and compares them to the third trimester placenta, providing valuable insights into the cellular composition, differentiation trajectories, and regulatory landscape of early placental development.Utilizing single-cell transcriptomics, we identified three distinct syncytiotrophoblast subtypes in the first trimester placenta, each characterized by unique gene expression profiles and marker genes. We further created bulk open chromatin maps to identify active transcription factor motifs within different placental cell types and subtypes. By analyzing genetic variation and CNVs within the placental genome, we also gained insights into the potential influence of structural genomic alterations on the placental regulatory landscape and gene expression patterns.Our findings provide a comprehensive understanding of the modality within the placenta during the late first trimester, as placental cells adeptly respond to the establishment of maternal blood supply, facilitating nutrient and oxygen transfer to the developing fetus. The integration of single-cell transcriptomics, epigenomics, and CNV analysis in this study enhances our understanding of the intricate processes underlying placental development. These findings contribute to the broader knowledge of the placenta's critical role in pregnancy and its implications for maternal and fetal health. Moreover, the identification of specific CNVs and their integration with transcriptional and epigenetic regulation opens avenues for future investigations into the functional consequences of genetic variation, pregnancy complications, and potential therapeutic interventions.

Subject Added Entry-Topical Term  

DNA methylation.

Subject Added Entry-Topical Term  

Enrollments.

Subject Added Entry-Topical Term  

Fetuses.

Subject Added Entry-Topical Term  

Patients.

Subject Added Entry-Topical Term  

Placenta.

Subject Added Entry-Topical Term  

Gene expression.

Subject Added Entry-Topical Term  

Stroke.

Subject Added Entry-Topical Term  

Fibroblasts.

Subject Added Entry-Topical Term  

Mutation.

Subject Added Entry-Topical Term  

Labeling.

Subject Added Entry-Topical Term  

Chromosomes.

Subject Added Entry-Topical Term  

Pregnancy.

Subject Added Entry-Topical Term  

Homogenization.

Subject Added Entry-Topical Term  

Genomes.

Subject Added Entry-Topical Term  

Hispanic people.

Subject Added Entry-Topical Term  

Epigenetics.

Subject Added Entry-Topical Term  

Ethnicity.

Subject Added Entry-Topical Term  

Genomics.

Subject Added Entry-Topical Term  

Gestational age.

Subject Added Entry-Topical Term  

Cell cycle.

Subject Added Entry-Topical Term  

Transcription factors.

Subject Added Entry-Topical Term  

Nitrogen.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Bioinformatics.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Ethnic studies.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Medicine.

Subject Added Entry-Topical Term  

Obstetrics.

Added Entry-Corporate Name  

Stanford University.

Host Item Entry  

Dissertations Abstracts International. 85-04A.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

로그인을 한후 보실 수 있는 자료입니다.

Control Number  

joongbu:642157