자료유형  

 학위논문

Control Number  

0016931860

International Standard Book Number  

9798379712129

Dewey Decimal Classification Number  

660

Main Entry-Personal Name  

Singh, Rohit Ranjan.

Publication, Distribution, etc. (Imprint  

[S.l.] : Cornell University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(292 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

General Note  

Advisor: Daniel, Susan.

Dissertation Note  

Thesis (Ph.D.)--Cornell University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Supported lipid bilayers (SLBs) have been used to characterize biological phenomena in membranes and to engineer those phenomena for applications such as sensing and separations. While microfluidic SLB platforms have developed considerably recently, extending the capabilities of such platforms for use in mimicking and engineering enzymatic reactions requires progress in a few different areas. These areas include an understanding of transport phenomena in lipid bilayers, the incorporation of transmembrane proteins into SLBs, the fluidization of those transmembrane proteins, and the spatial patterning of SLBs containing different biological materials. Some of the progress made in these areas is described here.First, improvements in SLB technologies are described. A platform in which material from outer membrane vesicles of Escherichia coli cells is incorporated into a SLB is characterized. The activity of an antibacterial compound on the SLB is consistent with previous studies of its activity on the outer membrane, showing that the platform recapitulates important features of the outer membrane. Next, the partial fluidization of transmembrane proteins in supported bilayers formed from mammalian cell blebs is described. The orientation and diffusion of proteins within these bilayers are characterized.Next, computational studies of diffusion of transmembrane proteins are described. The long-time diffusivity of a single transmembrane protein interacting with a random array of immobilized transmembrane proteins via both hydrodynamic and thermodynamic interactions is characterized. Next, the same model is used to characterize the effect of immobile proteins on the diffusive mode of a single mobile protein. Results from these two studies appear to be consistent with experimental work. Afterwards, a Brownian dynamics simulation method is described which includes 2D hydrodynamic interactions relevant for transmembrane proteins.Finally, efforts to use SLBs to engineer enzymatic reactions in a defined sequence are described. The transfer of a glycan from a lipid scaffold to a soluble protein using a transmembrane enzyme embedded in a SLB is demonstrated. Progress in synthesizing the ganglioside GM1 via sequential enzymatic addition of two sugar monomers is then described. In conclusion, additional steps needed to achieve the development of a microfluidic SLB platform to carry out sequential enzymatic reactions are discussed.

Subject Added Entry-Topical Term  

Chemical engineering.

Subject Added Entry-Topical Term  

Biophysics.

Subject Added Entry-Topical Term  

Bioengineering.

Index Term-Uncontrolled  

Transmembrane proteins

Index Term-Uncontrolled  

Supported lipid bilayers

Index Term-Uncontrolled  

Synthetic biomembrane platforms

Index Term-Uncontrolled  

SLB technologies

Index Term-Uncontrolled  

Enzymatic reactions

Added Entry-Corporate Name  

Cornell University Chemical Engineering

Host Item Entry  

Dissertations Abstracts International. 84-12B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:642086