Material Type  

 학위논문

 

0016931295

Date and Time of Latest Transaction  

20240214095955

ISBN  

9798380622585

DDC  

610

Author  

Cotner, Kristen.

Title/Author  

Integrated Node-Pore Sensing Techniques for Label-Free Single-Cell Measurements - [electronic resource]

Publish Info  

[S.l.] : University of California, Berkeley., 2022

Publish Info  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Material Info  

1 online resource(115 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

General Note  

Advisor: Sohn, Lydia.

학위논문주기  

Thesis (Ph.D.)--University of California, Berkeley, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Abstracts/Etc  

요약Microfluidic devices that can analyze single cells at high throughput are powerful tools in biomedical research, and many such technologies have been developed in recent years. Nodepore sensing (NPS) is one such platform, and NPS devices have been developed that measure a variety of both physical and molecular cell properties. The NPS platform benefits from being inherently label-free, meaning that cells do not need to be tagged with fluorescent or magnetic labels. Additionally, as NPS relies on an entirely electronic measurement, it is a robust system that is relatively low-cost to implement. These factors make NPS an attractive platform for biomedical research because it is accessible and easy to use while enabling multi-parameter single-cell measurements. This platform has already led to several new biological discoveries, although it still has some technical limitations.This dissertation focuses on characterizing the NPS platform and extending its technical capabilities. First, we present a quantitative study demonstrating the user-friendliness and reliability of our custom NPS data processing software. Second, we describe the theoretical framework and experimental characterization of a novel method to achieve multichannel NPS measurements for the first time. Finally, we introduce a new NPS platform with the unique ability to combine single-cell label-free mechanical and biomolecular measurements. These advancements pave the way toward next-generation, fully integrated NPS devices that can provide new insight into biologically and clinically important questions.

Subject Added Entry-Topical Term  

Bioengineering.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Oncology.

Index Term-Uncontrolled  

Breast cancer

Index Term-Uncontrolled  

Microfluidics

Index Term-Uncontrolled  

Node-pore sensing

Index Term-Uncontrolled  

Resistive pulse sensing

Index Term-Uncontrolled  

Single-cell

Index Term-Uncontrolled  

Biomolecular measurements

Added Entry-Corporate Name  

University of California, Berkeley Bioengineering

Host Item Entry  

Dissertations Abstracts International. 85-04B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

로그인을 한후 보실 수 있는 자료입니다.

소장사항  

202402 2024

Control Number  

joongbu:641930