자료유형  

 학위논문

Control Number  

0016935606

International Standard Book Number  

9798380372831

Dewey Decimal Classification Number  

610

Main Entry-Personal Name  

Stephens, Andrew D.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of Michigan., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(125 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

General Note  

Advisor: Kurabayashi, Katsuo.

Dissertation Note  

Thesis (Ph.D.)--University of Michigan, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Restrictions on Access Note  

This item must not be added to any third party search indexes.

Summary, Etc.  

요약Precision medicine is transforming healthcare from "one-size-fits-all" diagnosis and treatment, toward individualized therapies based on a person's genomics, phenomics, and environment. It has been enabled largely by technological improvements in analytical tools used to collect these patient-specific data. As precision medicine expands into more fields of healthcare, demands increase for new technologies to support it. Microfluidics biosensing technologies offer significant potential for this purpose, but there is a lack of scalable manufacturing methods to translate these technologies into clinical use. To address this gap, this thesis has developed methods of converting microfluidics technologies made with conventional materials to materials compatible with high volume manufacturing technologies, specifically, wafer-level batch microfabrication.In the first part of the thesis, a fabrication process for a microfluidic cell sorter and incubator was developed using standard silicon-on-insulator (SOI) microfabrication methods. This work advanced the capabilities of cellular functional immunophenotyping by enabling sufficient device manufacturing throughput to examine a matrix of stimulation conditions that could be used to determine an optimal dose of an immunosuppressive drug in vitro.In the second part, a new method of micro-patterning proteins is developed for coating antibodies within hydrophilic-in-hydrophobic microwells. This bio-compatible fabrication method enabled the scale-down of the sensor area required for the "digital enzyme-linked immunosorbent assay (ELISA)", capable of single-molecule detection. This enabled the construction of a highly sensitive and sample-sparing "digital protein microarray", which enabled the longitudinal study of six serum cytokines related to glioma tumor progression in a cohort of mice at two time points without pooling samples or sacrificing animal models.In the third and final part, technical, practical, and economical bottlenecks for clinical translation of microfluidics biosensing technologies are identified through interviewing over 130 potential end users of the technologies developed in thesis. The findings of these interviews serve as case study for other microfluidics researchers developing clinical diagnostic technologies, which will inform critical technical decisions made early in the research process to maximize future societal impact.The technologies developed and findings presented have addressed the long-standing issues of manufacturing and clinical translation in over 30 years of research by the microfluidics community. The thesis will potentially accelerate the lab-to-application pipeline for microfluidics technologies urgently needed for the future of healthcare.

Subject Added Entry-Topical Term  

Biomedical engineering.

Subject Added Entry-Topical Term  

Mechanical engineering.

Subject Added Entry-Topical Term  

Medicine.

Index Term-Uncontrolled  

Microfluidics

Index Term-Uncontrolled  

Clinical translation

Index Term-Uncontrolled  

Microfabrication methods

Index Term-Uncontrolled  

Precision medicine

Index Term-Uncontrolled  

Immunoassay

Added Entry-Corporate Name  

University of Michigan Mechanical Engineering

Host Item Entry  

Dissertations Abstracts International. 85-03B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641772