자료유형  

 학위논문

Control Number  

0016932740

International Standard Book Number  

9798379843175

Dewey Decimal Classification Number  

612

Main Entry-Personal Name  

Auber, Robert P.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2022

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Physical Description  

1 online resource(122 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-01, Section: B.

General Note  

Advisor: Wisecaver, Jennifer H.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Specialized metabolites are chemical tools produced by organisms to aid in their interaction with the surrounding environment. These diverse compounds can often function as metabolic weapons (e.g. antibiotics), structural components (e.g. lignins), or even attractants (e.g. flavonoids). Because of their frequent utilization in niche environments, specialized metabolite production is often lineage- or even species-specific. Therefore, knowledge between specialized metabolic systems is often nontransferable, which poses a major obstacle in the characterization of these bioactive and commercially relevant compounds. Beyond resolving the chemical composition of a specialized metabolite, the identification of responsible pathway genes and the evolutionary processes responsible for their formation is an arduous task. These gaps in knowledge are further widened by the lack of genomic resources available for specialized metabolite producing species. In this work, we present the genome assemblies of two organisms, each with unique specialized metabolic pathways: the Chinese medicinal plant Lithospermum erythrorhizon and the toxic golden alga Prymnesium parvum. Leveraging the predicted proteome of L. erythrorhizon, we investigated the evolutionary history of specialized metabolic genes responsible for the production of shikonin, a 1,4-naphthoquinone specialized metabolite. We identified a retrotranspositionmediated duplication event responsible for the creation of the core shikonin biosynthesis gene, PGT. In addition, we performed a global coexpression network analysis to identify regulatory and enzymatic gene candidates involved in the shikonin biosynthesis pathway. We also built phylogenetic trees of known and candidate shikonin genes to reveal patterns of lineage-specific gene duplication and retroduplication. Like plants, unicellular algae are known for their production of diverse, often toxic, specialized metabolites. However, these species are often enigmatic. For example, previous studies have documented large phenotypic variation in both toxin chemotypes and levels among different strains of P. parvum. To investigate the genetic basis of this variation, we generated near chromosome level assemblies of two P. parvumstrains and performed a broad genome survey of thirteen additional strains. As a result, we identified a commonly studied reference strain, UTEX 2797, as a hybrid with two distinct subgenomes. We also provide evidence of significant variation in haploid genome size across the species. Collectively, these studies supply genetic resources for the future study of these organisms, as well as provide insight into the evolution of their specialized metabolic pathways.

Subject Added Entry-Topical Term  

Physiology.

Subject Added Entry-Topical Term  

Fungi.

Subject Added Entry-Topical Term  

Phylogenetics.

Subject Added Entry-Topical Term  

Evolution.

Subject Added Entry-Topical Term  

Biosynthesis.

Subject Added Entry-Topical Term  

Mutation.

Subject Added Entry-Topical Term  

Genomes.

Subject Added Entry-Topical Term  

Metabolism.

Subject Added Entry-Topical Term  

Ribosomal DNA.

Subject Added Entry-Topical Term  

Genomics.

Subject Added Entry-Topical Term  

Horticulture.

Subject Added Entry-Topical Term  

Genetic diversity.

Subject Added Entry-Topical Term  

Gene expression.

Subject Added Entry-Topical Term  

Herbal medicine.

Subject Added Entry-Topical Term  

Genetic engineering.

Subject Added Entry-Topical Term  

Taxonomy.

Subject Added Entry-Topical Term  

Enzymes.

Subject Added Entry-Topical Term  

Metabolites.

Subject Added Entry-Topical Term  

Transcription factors.

Subject Added Entry-Topical Term  

Alternative medicine.

Subject Added Entry-Topical Term  

Bioengineering.

Subject Added Entry-Topical Term  

Bioinformatics.

Subject Added Entry-Topical Term  

Evolution & development.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Medicine.

Subject Added Entry-Topical Term  

Systematic biology.

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 85-01B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

로그인을 한후 보실 수 있는 자료입니다.

Control Number  

joongbu:641688