자료유형  

 학위논문

Control Number  

0016932662

International Standard Book Number  

9798379833824

Dewey Decimal Classification Number  

600

Main Entry-Personal Name  

Britton, Brianna Colleen.

Publication, Distribution, etc. (Imprint  

[S.l.] : Purdue University., 2021

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2021

Physical Description  

1 online resource(124 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-01, Section: A.

General Note  

Advisor: Oliver, Haley F.

Dissertation Note  

Thesis (Ph.D.)--Purdue University, 2021.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Foodborne illnesses have global impacts and research institutions, government agencies, and the private sector have made significant effortsto understand the causative agents of foodborne illness and to discover new ways to combat them. There are a number of foodborne pathogens of interest (e.g. Escherichia coli, Salmonella spp., Campylobacter spp., and Listeria monocytogenes) and other microorganisms that impact food safety and security on a global scale. Additionally, COVID-19 was declared a global pandemic in March 2020; the pandemic greatly impacted research efforts for more than a year. This dissertation discusses three different studies that highlight my contribution to the efforts to mitigate foodborne illness both domestically and abroad. The chapters of this dissertation describe (i) the impact of COVID-19 on Listeria monocytogenesin retail deli departments, (ii) the microbial consortia inhabiting retail deli drains and drain biofilms, and (iii) microbial contamination of peanuts produced and sold in the Senegalese peanut basin.Chapter 2:"Listeria monocytogenes prevalence in retail delicatessen departments decreased during the first year of the COVID-19 pandemic" describes a study investigating L. monocytogenes contamination in retail deli departments during COVID-19 and validating a predictive risk model associated with the enhanced cleaning and sanitation procedures utilized in response to the pandemic. This study was conducted in 44 retail deli departments across seven states in the US. The results showed that L. monocytogenes prevalence decreased from 5.8% positive prior to March 2020 to 4.3% during the pandemic. No L. monocytogenes was found on the scales or trashcans, which were factors previously correlated with high L. monocytogenes prevalence (10%). The predictive model accurately predicted high L. monocytogenes in 10/17 stores with high prevalence (α 0.0001, β=0.1186) during the COVID-19 pandemic. Cleaning and sanitation protocols were the factors most highly correlated with highL. monocytogenes prevalence in the conducted survey. These results indicated that the heightened awareness of personal hygiene and cleaning and sanitation due to COVID-19 likely reduced the prevalence of L. monocytogenesin retail delis.Chapter 3: "Characterization of retail delicatessen drains and biofilms using 16S rRNA metataxonomic and shotgun metagenomic sequencing" was a study designed to understand the microbial ecology of retail deli drains and biofilms harvested from deli drains. In this study, 14 biofilms were harvested from drain trenches and environmental sponge samples were collected from the surface of the same drain covers. 16S rRNA gene sequencing was used to characterize the microbiome of the biofilms and sponge samples and shotgun metagenomics analysis was conducted on nine biofilms with ≥10 ng/µL DNA. While Pseudomonas spp. dominated the microbiomes of the biofilms and drain surfaces, the microbial consortia inhabiting each location of the drains was vastly different. Additionally, shotgun metagenomics revealed that pathogenic bacterial species were in low abundance in the biofilms, and rare taxa reside in the same biofilms. Common sanitizer resistance genes (qacE∆1, qacE, and qacL) were observed in the biofilms as well, indicating possible increased tolerance to quaternary ammonium-based sanitizers.Chapter 4: "Microbial contamination patterns in peanuts produced and sold in the Senegalese peanut basin" was a study conducted as part of the USAID Feed the Future Food Safety Innovation Lab (FSIL) in collaboration with the Senegalese Institute of Agricultural Research (ISRA). A survey was conducted of 198 households that produce peanuts in Senegal and 198 peanut samples were collected for microbial analysis. These peanut samples were evaluated for Enterobacteriaceae, coliforms, and total yeast and mold concentrations, then observed populations (log CFU/g) were correlated with survey questions related to producer knowledge of microbial contamination and storage methods utilized by producers. The results indicated that peanuts were heavily contaminated with the fecal indicators Enterobacteriaceae and coliforms (13.0% and 13.6% above detection limit [5.0 log CFU/g], respectively). Only 22.7% and 18.7% of producers reported they had heard of pathogenic bacteria or aflatoxins, respectively, before this study. Additionally, the combination of storage container type and whether the peanuts were store off the ground were predictive of bacterial contamination. This study provides preliminary data to inform future studies which should assess prevalence of pathogenic microorganisms (e.g. Salmonella spp. and E. coli) and evaluate preventive measures to be utilized during harvest and storage to minimize the risk of microbial contamination of peanuts.

Subject Added Entry-Topical Term  

Pathogens.

Subject Added Entry-Topical Term  

Womens health.

Subject Added Entry-Topical Term  

Livestock.

Subject Added Entry-Topical Term  

Food products.

Subject Added Entry-Topical Term  

Food contamination & poisoning.

Subject Added Entry-Topical Term  

Salmonella.

Subject Added Entry-Topical Term  

Biofilms.

Subject Added Entry-Topical Term  

Bacteria.

Subject Added Entry-Topical Term  

Meningitis.

Subject Added Entry-Topical Term  

E coli.

Subject Added Entry-Topical Term  

Genes.

Subject Added Entry-Topical Term  

COVID-19.

Subject Added Entry-Topical Term  

Ecology.

Subject Added Entry-Topical Term  

Illnesses.

Subject Added Entry-Topical Term  

Microorganisms.

Subject Added Entry-Topical Term  

Peanuts.

Subject Added Entry-Topical Term  

Disease transmission.

Subject Added Entry-Topical Term  

Meat processing.

Subject Added Entry-Topical Term  

Animal sciences.

Subject Added Entry-Topical Term  

Finance.

Subject Added Entry-Topical Term  

Food science.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Public health.

Subject Added Entry-Topical Term  

Sub Saharan Africa studies.

Added Entry-Corporate Name  

Purdue University.

Host Item Entry  

Dissertations Abstracts International. 85-01A.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641507