자료유형  

 학위논문

Control Number  

0016931298

International Standard Book Number  

9798380623087

Dewey Decimal Classification Number  

574.191

Main Entry-Personal Name  

Herrera Asmat, Omar Carlos.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of California, Berkeley., 2022

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2022

Physical Description  

1 online resource(78 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

General Note  

Advisor: Bustamante, Carlos.

Dissertation Note  

Thesis (Ph.D.)--University of California, Berkeley, 2022.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Tuberculosis (TB) is an airborne disease that kills 1.5 million people and affects ~ 9 million worldwide. Its etiological agent, Mycobacterium tuberculosis (Mtb), persists in a third of the general population. There is also an emergent problem of drug and multidrug resistance in Mtb that creates the need for novel drugs. In the treatment against TB, one target of antibiotics is the prokaryotic RNA polymerase (RNAP), an essential multitask enzyme that catalyzes RNA synthesis during transcription. Rifampicin, the first-line antibiotic anti-TB, binds and inhibits the MtbRNAP.Consequently, combatting TB requires a better understanding of the primary mechanism of transcription by MtbRNAP. In fact, during Mtb transcription, unique and essential factors might affect the MtbRNAP dynamics and regulate its activity. That regulation plays a significant role in the adaptation and pathogenesis of the parasite. Therefore, understanding how these factors and new antibiotics modulate together the MtbRNAP activity will contribute valid information to address TB. To this end, we use a single-molecule approach based on high-resolution optical tweezers to study transcription elongation by MtbRNAP in the context of its DNA elements, transcriptional factors, and antibiotics.

Subject Added Entry-Topical Term  

Biophysics.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Antibiotic

Index Term-Uncontrolled  

Drug

Index Term-Uncontrolled  

Mycobacterium tuberculosis

Index Term-Uncontrolled  

Optical tweezers

Index Term-Uncontrolled  

Single molecule

Added Entry-Corporate Name  

University of California, Berkeley Molecular & Cell Biology

Host Item Entry  

Dissertations Abstracts International. 85-04B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641441