자료유형  

 학위논문

Control Number  

0016934080

International Standard Book Number  

9798380134187

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Brown, Nolan.

Publication, Distribution, etc. (Imprint  

[S.l.] : The University of North Carolina at Chapel Hill., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(71 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-02, Section: B.

General Note  

Advisor: Erie, Dorothy;Brustad, Eric.

Dissertation Note  

Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Unnatural amino acid (UAA) technology offers powerful and flexible tools for addressing many issues in human health, scientific research, and industry. However, UAA technology is currently limited by prohibitive costs and poor availability. Evolution of UAA biosynthetic enzymes can make UAAs much cheaper and more available, but previous efforts to evolve or design UAA biosynthetic enzymes have only used low-throughput methods which are limiting for general use. This thesis presents a framework by which large libraries of mutant UAA biosynthetic enzymes can be generated for truly high-throughput selections. This thesis also describes the generation of chloramphenicol resistance and GFP selection marker genetic constructs optimized for performing selections on large libraries of UAA biosynthetic enzymes. The combination of this library generation framework and selection markers optimized for finding UAA biosynthetic enzymes should greatly facilitate the evolution of UAA biosynthetic enzymes and drastically improve the cost and availability of UAA technology.

Subject Added Entry-Topical Term  

Biochemistry.

Subject Added Entry-Topical Term  

Genetics.

Subject Added Entry-Topical Term  

Biology.

Subject Added Entry-Topical Term  

Molecular biology.

Index Term-Uncontrolled  

Amino acid

Index Term-Uncontrolled  

Biosynthetic enzymes

Index Term-Uncontrolled  

Flexible tools

Index Term-Uncontrolled  

Chloramphenicol resistance

Index Term-Uncontrolled  

Genetic constructs

Added Entry-Corporate Name  

The University of North Carolina at Chapel Hill Genetics and Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 85-02B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:641159