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Genetically Engineered Bacteriophages for Biotechnology and Biomedicine Applications- [electronic resource]
Genetically Engineered Bacteriophages for Biotechnology and Biomedicine Applications- [electronic resource]
상세정보
- 자료유형
- 학위논문
- Control Number
- 0016931570
- International Standard Book Number
- 9798379710651
- Dewey Decimal Classification Number
- 641
- Main Entry-Personal Name
- Carmody, Caitlin Marie.
- Publication, Distribution, etc. (Imprint
- [S.l.] : Cornell University., 2023
- Publication, Distribution, etc. (Imprint
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- Physical Description
- 1 online resource(277 p.)
- General Note
- Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
- General Note
- Advisor: Nugen, Sam.
- Dissertation Note
- Thesis (Ph.D.)--Cornell University, 2023.
- Restrictions on Access Note
- This item must not be sold to any third party vendors.
- Summary, Etc.
- 요약Bacteriophages' abundance and high specificity to infecting only bacteria hosts within their narrow host range have made them an ideal candidate for utilization in bacteria diagnostics and bacterial infection targeting therapeutics. Genetic and chemical modifications have been performed on bacteriophages to functionalize them with a variety of molecules including fluorophores, antigens, nanoparticles, drugs, and polymers to tailor their features for specific applications. In this work, the genetic engineering tool CRISPR-Cas9 was employed to facilitate modification of the large and complex genome of bacteriophage T4. This bacteriophage is well-characterized and infects Escherichia coli hosts making it an ideal candidate for modeling bacteriophage modifications. Throughout this work, T4 bacteriophages were genetically modified to functionalize them with luciferases for quantifiable signal production, affinity peptides for immobilization and conjugation, and chimeric tail fibers for altering host range. A diagnostic assay capable of detecting 10 CFU of E. coli in 100mLs of water in low-resource settings was developed utilizing one of the modified T4 bacteriophages as a biosensor. The genetic and phenotypic information gathered in this work can be applied to bacteriophages that infect bacteria strains prominent in agriculture and pharmaceutical industries to advance bacteriophage biotechnologies and biomedicines.
- Subject Added Entry-Topical Term
- Food science.
- Subject Added Entry-Topical Term
- Genetics.
- Subject Added Entry-Topical Term
- Bioengineering.
- Index Term-Uncontrolled
- Bacteriophage
- Index Term-Uncontrolled
- Biopharma
- Index Term-Uncontrolled
- Biotechnology
- Index Term-Uncontrolled
- Genetic engineering
- Index Term-Uncontrolled
- Microbiology
- Added Entry-Corporate Name
- Cornell University Food Science and Technology
- Host Item Entry
- Dissertations Abstracts International. 84-12B.
- Host Item Entry
- Dissertation Abstract International
- Electronic Location and Access
- 로그인을 한후 보실 수 있는 자료입니다.
- Control Number
- joongbu:640040
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798379710651
■035 ▼a(MiAaPQ)AAI30314727
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a641
■1001 ▼aCarmody, Caitlin Marie.▼0(orcid)0000-0002-2295-1427
■24510▼aGenetically Engineered Bacteriophages for Biotechnology and Biomedicine Applications▼h[electronic resource]
■260 ▼a[S.l.]▼bCornell University. ▼c2023
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2023
■300 ▼a1 online resource(277 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 84-12, Section: B.
■500 ▼aAdvisor: Nugen, Sam.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2023.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aBacteriophages' abundance and high specificity to infecting only bacteria hosts within their narrow host range have made them an ideal candidate for utilization in bacteria diagnostics and bacterial infection targeting therapeutics. Genetic and chemical modifications have been performed on bacteriophages to functionalize them with a variety of molecules including fluorophores, antigens, nanoparticles, drugs, and polymers to tailor their features for specific applications. In this work, the genetic engineering tool CRISPR-Cas9 was employed to facilitate modification of the large and complex genome of bacteriophage T4. This bacteriophage is well-characterized and infects Escherichia coli hosts making it an ideal candidate for modeling bacteriophage modifications. Throughout this work, T4 bacteriophages were genetically modified to functionalize them with luciferases for quantifiable signal production, affinity peptides for immobilization and conjugation, and chimeric tail fibers for altering host range. A diagnostic assay capable of detecting 10 CFU of E. coli in 100mLs of water in low-resource settings was developed utilizing one of the modified T4 bacteriophages as a biosensor. The genetic and phenotypic information gathered in this work can be applied to bacteriophages that infect bacteria strains prominent in agriculture and pharmaceutical industries to advance bacteriophage biotechnologies and biomedicines.
■590 ▼aSchool code: 0058.
■650 4▼aFood science.
■650 4▼aGenetics.
■650 4▼aBioengineering.
■653 ▼aBacteriophage
■653 ▼aBiopharma
■653 ▼aBiotechnology
■653 ▼aGenetic engineering
■653 ▼aMicrobiology
■690 ▼a0359
■690 ▼a0369
■690 ▼a0202
■71020▼aCornell University▼bFood Science and Technology.
■7730 ▼tDissertations Abstracts International▼g84-12B.
■773 ▼tDissertation Abstract International
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16931570▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
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