자료유형  

 학위논문

Control Number  

0016931826

International Standard Book Number  

9798379750848

Dewey Decimal Classification Number  

574

Main Entry-Personal Name  

Pather, Sarshan Rubintheran.

Publication, Distribution, etc. (Imprint  

[S.l.] : University of Pennsylvania., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(192 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

General Note  

Advisor: Shalem, Ophir;Song, Hongjun.

Dissertation Note  

Thesis (Ph.D.)--University of Pennsylvania, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Unraveling the complexity of human brain function demands integrated profiling, generation, and creative disruption of cell type-specific states to decode genotype-phenotype relationships. Here, I present a thesis tackling each of these areas. Profiling or "reading" of human brain tissue at the single cell level with technical innovations in transcriptomics provides deep molecular and cellular reference maps describing the landscape of human neural cell types. Generation or "writing" of synthetic human neural tissue using emerging human induced pluripotent stem cell differentiation technologies provides tractable cellular models to study human-specific neural cell biology. Disruption or "perturbing" of human neural tissue with pooled genome editing CRISPR screening systems enables scalable discovery of novel genotype-phenotype relationships. Continued work in these areas will enhance our understanding of how human neural phenotypes shift from healthy to diseased states and inform the development of new therapeutics and diagnostics to drive human neural phenotypes from diseased to healthy states.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Molecular biology.

Subject Added Entry-Topical Term  

Neurosciences.

Index Term-Uncontrolled  

In situ sequencing

Index Term-Uncontrolled  

Induced pluripotent stem cells

Index Term-Uncontrolled  

Neural organoids

Index Term-Uncontrolled  

Optical screening

Index Term-Uncontrolled  

Single cell biology

Added Entry-Corporate Name  

University of Pennsylvania Cell and Molecular Biology

Host Item Entry  

Dissertations Abstracts International. 84-12B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

로그인을 한후 보실 수 있는 자료입니다.

Control Number  

joongbu:639510