자료유형  

 학위논문

Control Number  

0016933192

International Standard Book Number  

9798380314879

Dewey Decimal Classification Number  

530

Main Entry-Personal Name  

Yu, Yue.

Publication, Distribution, etc. (Imprint  

[S.l.] : Cornell University., 2023

Publication, Distribution, etc. (Imprint  

Ann Arbor : ProQuest Dissertations & Theses, 2023

Physical Description  

1 online resource(117 p.)

General Note  

Source: Dissertations Abstracts International, Volume: 85-03, Section: B.

General Note  

Advisor: Kourkoutis, Lena.

Dissertation Note  

Thesis (Ph.D.)--Cornell University, 2023.

Restrictions on Access Note  

This item must not be sold to any third party vendors.

Summary, Etc.  

요약Cryo-electron microscopy (cryo-EM) is a powerful technique extensively utilized for studying biological systems. It allows for high-resolution structural determination of macromolecules and visualization of cellular structures in their near-native environment. This thesis starts with a concise overview of the fundamental principles and applications of selective cryo-EM techniques with conventional transmission electron microscopy (TEM), such as single particle analysis (cryo-SPA), electron tomography (cryo-ET), and focused-ion-beam milling (cryo-FIB). Subsequently, applications of scanning-TEM (STEM) in vitrified hydrated biological systems are introduced, along with the progress made in four-dimensional STEM (4DSTEM) techniques.The challenges encountered in imaging thick biological specimens using conventional TEM techniques are highlighted, along with the limited applicability of conventional STEM for high-resolution structural determination due to collection efficiency limitations. To address these challenges, a 4D-STEM technique, tilt-corrected bright-field STEM (tcBF-STEM) is introduced, aiming to provide advantages for imaging thick dose-sensitive specimens by optimizing the collection efficiency of coherent BF-STEM images. The implementation of tcBF-STEM with a pixelated detector optimized for STEM is demonstrated, along with the extraction of probe aberration information using tcBF imaging. Notably, tcBF-STEM enables the retrieval of information beyond the real-space scanning Nyquist limit, effectively mitigating the effects of specimen motion in cryo-EM experiments with a single tilt direction. The results of applying tcBFSTEM to imaging vitrified biological specimens, including vitrified apoferritin protein and E. coli bacteria, are presented. The potential of tcBF-STEM and its limitations are discussed at the end.

Subject Added Entry-Topical Term  

Applied physics.

Subject Added Entry-Topical Term  

Microbiology.

Subject Added Entry-Topical Term  

Cellular biology.

Subject Added Entry-Topical Term  

Biophysics.

Index Term-Uncontrolled  

Apoferritin

Index Term-Uncontrolled  

Cryogenic electron microscopy

Index Term-Uncontrolled  

Escherichia coli

Index Term-Uncontrolled  

Four-dimensional STEM

Index Term-Uncontrolled  

Vitrified biological specimens

Index Term-Uncontrolled  

Transmission electron microscopy

Added Entry-Corporate Name  

Cornell University Applied Physics

Host Item Entry  

Dissertations Abstracts International. 85-03B.

Host Item Entry  

Dissertation Abstract International

Electronic Location and Access  

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Control Number  

joongbu:639441